Crutcher K A, Weingartner J
Department of Neurosurgery, University of Cincinnati, College of Medicine, OH.
Neurobiol Aging. 1991 Sep-Oct;12(5):449-54. doi: 10.1016/0197-4580(91)90072-r.
The sympathetic sprouting response that occurs in the rat hippocampal formation following septal denervation is reduced in aged rats. Since considerable evidence implicates NGF-like activity in eliciting the sprouting, the simplest explanation for the age-related decline in sympathetic sprouting is a reduction in hippocampal NGF levels. In the present study, hippocampal NGF levels were measured using a 2-site ELISA in four different age groups of Fischer 344 rats. There was no decline in NGF levels with age, nor did we find any differences between male and female rats. This contradicts an earlier report in which a 40% reduction in hippocampal NGF protein levels was found in aged rats. Possible reasons for this discrepancy are discussed. The present results do not support the hypothesis that the age-related decline in sympathetic sprouting is due to a reduction in total hippocampal NGF levels.
在隔区去神经支配后大鼠海马结构中出现的交感神经芽生反应在老年大鼠中有所降低。由于大量证据表明类神经生长因子（NGF）活性参与引发芽生，因此交感神经芽生随年龄下降的最简单解释是海马NGF水平降低。在本研究中，使用双位点酶联免疫吸附测定法（ELISA）测量了四个不同年龄组的Fischer 344大鼠的海马NGF水平。NGF水平并未随年龄下降，我们也未发现雄性和雌性大鼠之间存在任何差异。这与早期的一份报告相矛盾，在该报告中发现老年大鼠海马NGF蛋白水平降低了40%。文中讨论了这种差异的可能原因。目前的结果不支持交感神经芽生随年龄下降是由于海马总NGF水平降低这一假说。
