用于药物筛选和毒素检测的细胞阻抗生物传感器。
Cellular impedance biosensors for drug screening and toxin detection.
作者信息
Asphahani Fareid, Zhang Miqin
机构信息
Department of Materials Science & Engineering, 302L Roberts Hall, University of Washington, Seattle, Washington 98195-2120, USA.
出版信息
Analyst. 2007 Sep;132(9):835-41. doi: 10.1039/b704513a. Epub 2007 Jun 26.
Abstract
Cell-based impedance biosensing is an emerging technology that can be used to non-invasively and instantaneously detect and analyze cell responses to chemical and biological agents. This article highlights the fabrication and measurement technologies of cell impedance sensors, and their application in toxin detection and anti-cancer drug screening. We start with an introduction that describes the capability and advantages of cell-based sensors over conventional sensing technology, followed by a discussion of the influence of cell adhesion, spreading and viability during cell patterning on the subsequent impedance measurements and sensing applications. We then present an electronic circuit that models the cell-electrode system, by which the cellular changes can be detected in terms of impedance changes of the circuit. Finally, we discuss the current status on using cell impedance sensors for toxin detection and anti-cancer drug screening.
摘要
基于细胞的阻抗生物传感是一项新兴技术，可用于非侵入性地即时检测和分析细胞对化学和生物制剂的反应。本文重点介绍了细胞阻抗传感器的制造和测量技术，以及它们在毒素检测和抗癌药物筛选中的应用。我们首先进行介绍，描述基于细胞的传感器相对于传统传感技术的能力和优势，接着讨论细胞图案化过程中细胞粘附、铺展和活力对后续阻抗测量和传感应用的影响。然后，我们展示一个对细胞 - 电极系统进行建模的电子电路，通过该电路的阻抗变化可以检测细胞变化。最后，我们讨论使用细胞阻抗传感器进行毒素检测和抗癌药物筛选的现状。
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