Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, CA 92697, USA.
Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA 92697, USA.
Biosensors (Basel). 2023 Dec 18;13(12):1036. doi: 10.3390/bios13121036.
Prostate cancer is the second leading cause of death in men. A challenge in treating prostate cancer is overcoming cell plasticity, which links cell phenotype changes and chemoresistance. In this work, a microfluidic device coupled with electrical impedance spectroscopy (EIS), an electrode-based cell characterization technique, was used to study the electrical characteristics of phenotype changes for (1) prostate cancer cell lines (PC3, DU145, and LNCaP cells), (2) cells grown in 2D monolayer and 3D suspension cell culture conditions, and (3) cells in the presence (or absence) of the anti-cancer drug nigericin. To validate observations of phenotypic change, we measured the gene expression of two epithelial markers, E-cadherin (CDH1) and Tight Junction Protein 1 (ZO-1). Our results showed that PC3, DU145, and LNCaP cells were discernible with EIS. Secondly, moderate phenotype changes based on differences in cell culture conditions were detected with EIS and supported by the gene expression of CDH1. Lastly, we showed that EIS can detect chemoresistant-related cell phenotypes with nigericin drug treatment. EIS is a promising label-free tool for detecting cell phenotype changes associated with chemoresistance. Further development will enable the detection and characterization of many other types of cancer cells.
前列腺癌是男性死亡的第二大主要原因。治疗前列腺癌的一个挑战是克服细胞可塑性，这将细胞表型变化和化疗耐药性联系起来。在这项工作中，使用了一种与电化学阻抗谱（EIS）相结合的微流控装置，EIS 是一种基于电极的细胞特征分析技术，用于研究（1）前列腺癌细胞系（PC3、DU145 和 LNCaP 细胞）、（2）在 2D 单层和 3D 悬浮细胞培养条件下生长的细胞、（3）以及在存在（或不存在）抗癌药物 Nigericin 的情况下的细胞的电特性变化。为了验证表型变化的观察结果，我们测量了两种上皮标志物 E-钙粘蛋白（CDH1）和紧密连接蛋白 1（ZO-1）的基因表达。我们的结果表明，EIS 可以区分 PC3、DU145 和 LNCaP 细胞。其次，EIS 检测到基于细胞培养条件差异的适度表型变化，并得到 CDH1 基因表达的支持。最后，我们表明 EIS 可以检测到 Nigericin 药物治疗相关的耐药相关细胞表型。EIS 是一种很有前途的无标记工具，可用于检测与化疗耐药相关的细胞表型变化。进一步的发展将能够检测和表征许多其他类型的癌细胞。
Zotero 插件
