Chen Yongfeng, Zhu Bide, Yan Suchun, Zhang Li, Li Jing
Department of Histology, Embryology and Neurobiology, School of Basic Medical Sciences of Sichuan University, Chengdu 610041, China.
J Tradit Chin Med. 2007 Jun;27(2):143-7.
To study the effect of weiganli ([Chinese characters: see text]) on bone marrow hemopoiesis.
The effects of weiganli on the peripheral blood picture and the number of bone marrow nucleated cells (BMCs) were observed in myelosuppressed anemic model mice, and the effects of weiganli on the growth of colony forming unit-granulocyte macrophage (CFU-GM), colony forming unit-erythroid (CFU-E), burst forming unit-erythroid (BFU-E), colony forming unit-megkaryocyte (CFU-Meg) were investigated by in vitro cell culture technique. The hemopoietic stem cells (HSCs, c-kit+) in bone marrow were double stained with fluorescent antibody PE-C-Kit and FITC-CD45, and the HSCs (c-kit+) were counted by flow cytometer with CD45/SSC (side scatter) gating.
Peripheral blood cell counts and the number of BMCs were significantly improved after weiganli administration; and bone marrow hemopoietic stem/progenitor cells were significantly increased.
Weiganli can effectively promote the recovery of hemopoietic function in the myelosuppressed anemic mice.
研究维肝灵对骨髓造血的影响。
观察维肝灵对骨髓抑制性贫血模型小鼠外周血象及骨髓有核细胞数量的影响，采用体外细胞培养技术研究维肝灵对粒-巨噬细胞集落形成单位（CFU-GM）、红系集落形成单位（CFU-E）、爆式红系集落形成单位（BFU-E）、巨核细胞集落形成单位（CFU-Meg）生长的影响。用荧光抗体PE-C-Kit和FITC-CD45对骨髓造血干细胞（HSCs，c-kit+）进行双重染色，通过流式细胞仪以CD45/SSC（侧向散射）设门对HSCs（c-kit+）进行计数。
给予维肝灵后外周血细胞计数及骨髓有核细胞数量显著改善；骨髓造血干/祖细胞显著增加。
维肝灵可有效促进骨髓抑制性贫血小鼠造血功能的恢复。
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