Fedorov N A, Veske M A, Ermil'chenko G V, Malykhina L S
Biull Eksp Biol Med. 1975 Sep;80(9):33-6.
The method of competitive protein binding was applied to the study of content of cyclic adenosine-3',5'-monophosphate (cAMP) in donor plasma one day before and immediately after the donation of 200 ml of blood. The work was performed by Gilman's radiochemical method. Two types of reaction of donors to donorship were revealed: without any changes and with the changes of the cAMP level. In accordance with these reactions the donors were divided into two subgroups--the stable and the reactive ones. In repeated donors the cAMP level was higher than in the primary ones, and the reactive ones. In repeated donors the cAMP level was higher than in the primary ones, and at the time of blood recovery it increased even more particularly among persons of "reactive" type. In primary donors of reactive type the cAMP content before the blood donation was either below or over the mean value and either increased or decreased to the mean level after the blood loss.
采用竞争性蛋白结合法，对200毫升献血前一天及献血后即刻的供血者血浆中3',5'-环磷酸腺苷（cAMP）含量进行了研究。研究工作采用吉尔曼的放射化学方法。结果显示，供血者对献血存在两种反应类型：cAMP水平无变化和有变化。根据这些反应，将供血者分为两个亚组——稳定型和反应型。多次献血者的cAMP水平高于初次献血者，且在恢复供血时，cAMP水平升得更高，尤其是“反应型”人群。在反应型初次献血者中，献血前cAMP含量要么低于要么高于平均值，失血后要么升高要么降低至平均水平。
