Chang Bing, Ge Junhui, Liang Yuxiang
Institute of Occupational Health and Poisoning Control, China CDC, Beijing 100050, China.
Wei Sheng Yan Jiu. 2007 May;36(3):259-62.
To studied the estrogenicity of single propylparaben (PP), butylparaben (BP), di-n-butyl phthalate (DBP), and their joint treatment.
Estrogenicity was studied using the uterotrophic assay of immature female Wistar rats. Animals were subcutaneously (sc) treated for three consecutive days with single PP, BP, DBP and their joint treatment. Estrogenic activity effects were identified through the ratio of uterus/body weight.
Estrogenic activities of PP and BP was detected, the lowest observed effect level (LOEL) of PP and BP were 400mg/kg and 200mg/ kg, respectively, estrogenic activity of DBP was not detected at the doses of 400mg/kg and 100mg/kg. In the equal effect dosage joint treatment of PP and BP, uterus proliferation effects were observed at the doses of 1 and 1/2 LOEL, but the effect was not observed at the doses of 1/4 LOEL. In the joint treatment with DBP increased uterus proliferation effects of PP or BP were observed at matches of 100mg/kg PP(1/4LOEL) + 400mg/kg DBP, 200mg/kg PP(1/2LOEL) + 400mg/kg DBP and 100mg/kg BP(1/2LOEL) + 400mg/kg DBP.
It was suggested that the DBP could have potential enhancing action to estrogenic activities of PP or BP, and the estrogenic activity of PP enhancement by DBP could be stronger than that of BP.
研究对羟基苯甲酸丙酯（PP）、对羟基苯甲酸丁酯（BP）、邻苯二甲酸二正丁酯（DBP）及其联合处理的雌激素活性。
采用未成熟雌性Wistar大鼠子宫增重试验研究雌激素活性。动物连续三天皮下注射处理单一的PP、BP、DBP及其联合处理物。通过子宫/体重比确定雌激素活性作用。
检测到PP和BP的雌激素活性，PP和BP的最低观察效应水平（LOEL）分别为400mg/kg和200mg/kg，在400mg/kg和100mg/kg剂量下未检测到DBP的雌激素活性。在PP和BP的等效剂量联合处理中，在1和1/2 LOEL剂量下观察到子宫增殖效应，但在1/4 LOEL剂量下未观察到该效应。在与DBP的联合处理中，在100mg/kg PP（1/4 LOEL）+ 400mg/kg DBP、200mg/kg PP（1/2 LOEL）+ 400mg/kg DBP和100mg/kg BP（1/2 LOEL）+ 400mg/kg DBP组合下观察到PP或BP的子宫增殖效应增强。
提示DBP可能对PP或BP的雌激素活性有潜在增强作用，且DBP对PP雌激素活性的增强作用可能强于BP。
