Jiang Haiyan, Du Jinlin, He Tao, Qu Jia, Song Zongming, Wu Shengzhou
School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China; State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China.
Clin Exp Ophthalmol. 2014 Dec;42(9):841-5. doi: 10.1111/ceo.12329. Epub 2014 May 8.
The objective of this clinical study is to examine the association between D-serine and diabetic retinopathy (DR).
Retrospective, case-control study was performed in the affiliated Eye Hospital of Wenzhou Medical University.
This study included 25 patients with proliferative diabetic retinopathy (PDR), and 25 sex- and age-matched control subjects, i.e. patients with idiopathic macular hole and idiopathic epiretinal membrane.
Clinical diagnoses were made by the senior ophthalmologists in the Eye Hospital; the aqueous and vitreous humour specimens were collected from these patients undergoing pars plana vitrectomy for treating complications.
The aqueous and vitreous levels of D-serine and glutamate were measured with reverse-phase high-performance liquid chromatography (HPLC); the contents of haemoglobin in the blood and in the vitreous specimens from PDR were measured with spectrophotometry to correct possible introduction of amino acids from PDR haemorrhage.
The concentrations of D-serine in the aqueous or vitreous humour were significantly higher in patients with PDR compared with control subjects. The vitreous concentrations of D-serine in PDR were 25.55 ± 0.63 μmol/L compared with control subjects at 22.76 ± 0.36 μmol/L (P = 0.002); the levels of D-serine in the aqueous humour from patients with PDR were 29.08 ± 1.31 μmol/L compared with control subjects at 24.22 ± 0.65 μmol/L (P = 0.006). Correction from possible introduction of D-serine from the vitreous haemorrhage in PDR did not significantly alter the findings.
Increased D-serine in the aqueous and vitreous humour was found in patients with PDR compared with control subjects.
本临床研究的目的是探讨D-丝氨酸与糖尿病视网膜病变（DR）之间的关联。
在温州医科大学附属眼视光医院进行回顾性病例对照研究。
本研究纳入25例增殖性糖尿病视网膜病变（PDR）患者，以及25例年龄和性别匹配的对照对象，即特发性黄斑裂孔和特发性视网膜前膜患者。
由眼视光医院的资深眼科医生进行临床诊断；从这些接受玻璃体切割术治疗并发症的患者中采集房水和玻璃体液标本。
采用反相高效液相色谱法（HPLC）测定房水和玻璃体液中D-丝氨酸和谷氨酸的水平；用分光光度法测定PDR患者血液和玻璃体液标本中的血红蛋白含量，以校正PDR出血可能引入的氨基酸。
与对照对象相比，PDR患者房水或玻璃体液中D-丝氨酸的浓度显著更高。PDR患者玻璃体液中D-丝氨酸的浓度为25.55±0.63μmol/L，而对照对象为22.76±0.36μmol/L（P = 0.002）；PDR患者房水中D-丝氨酸的水平为29.08±1.31μmol/L，对照对象为24.22±0.65μmol/L（P = 0.006）。校正PDR玻璃体出血可能引入的D-丝氨酸后，结果无显著改变。
与对照对象相比，PDR患者房水和玻璃体液中的D-丝氨酸增加。
