Liao I C, Chew S Y, Leong K W
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Nanomedicine (Lond). 2006 Dec;1(4):465-71. doi: 10.2217/17435889.1.4.465.
Continuous nanostructures embedded with proteins may synergistically present topographical and biochemical signals to cells for tissue engineering applications. This study presents the co-axial electrospinning of aligned poly(epsilon-caprolactone) nanofibers encapsulated with bovine serum albumin and platelet-derived growth factor-bb for demonstration of controlled release and bioactivity retention, respectively.
MATERIALS & METHODS: Controllable release kinetics is achieved by incorporation of poly(ethylene glycol) as a porogen in the shell of the nanofibers.
RESULTS & DISCUSSION: Poly(ethylene glycol) leaches out in a concentration- and molecular weight-dependent fashion, leading to bovine serum albumin release half-lives that range from 1 to 20 days. Optimized platelet-derived growth factor-bb-encapsulated nanofibers can completely release the protein with near zero-order kinetics and preserved bioactivity.
Co-axial electrospinning is shown to be a versatile technique in achieving the delivery of biochemical signals in a controlled manner for regenerative medicine applications.
嵌入蛋白质的连续纳米结构可协同向细胞呈现形貌和生化信号，用于组织工程应用。本研究展示了同轴静电纺丝制备包裹牛血清白蛋白和血小板衍生生长因子-bb的取向聚（ε-己内酯）纳米纤维，分别用于证明控释和生物活性保留。
通过在纳米纤维壳层中加入聚乙二醇作为致孔剂来实现可控的释放动力学。
聚乙二醇以浓度和分子量依赖的方式浸出，导致牛血清白蛋白的释放半衰期为1至20天。优化后的包裹血小板衍生生长因子-bb的纳米纤维能够以接近零级动力学完全释放蛋白质并保留生物活性。
同轴静电纺丝被证明是一种通用技术，可用于再生医学应用中以可控方式递送生化信号。
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