Akhmetova Alma, Lanno Georg-Marten, Kogermann Karin, Malmsten Martin, Rades Thomas, Heinz Andrea
LEO Foundation Center for Cutaneous Drug Delivery, Department of Pharmacy, University of Copenhagen, 2100 Copenhagen, Denmark.
Institute of Technology, University of Tartu, 50411 Tartu, Estonia.
Pharmaceutics. 2020 May 18;12(5):458. doi: 10.3390/pharmaceutics12050458.
The development of biomaterials for wound healing applications requires providing a number of properties, such as antimicrobial action, facilitation of cell proliferation, biocompatibility and biodegradability. The aim of the present study was to investigate morphological and mechanical properties of zein-based microfibers, ultimately aimed at creating an environment suitable for wound healing. This was achieved through co-axial electrospinning of core-shell microfibers, with zein protein in the core and polyethylene oxide (PEO) in the shell. Small amounts of PEO or stearic acid were additionally incorporated into the fiber core to modify the morphology and mechanical properties of zein fibers. The presence of PEO in the core was found to be essential for the formation of tubular fibers, whereas PEO in the shell enhanced the stability of the microfibers in water and ensured high elasticity of the microfiber mats. Tetracycline hydrochloride was present in an amorphous form within the fibers, and displayed a burst release as a result of pore-formation in the fibers. The developed systems exhibited antimicrobial activity against and , and showed no cytotoxic effect on fibroblasts. Biocompatibility, antimicrobial activity and favorable morphological and mechanical properties make the developed zein-based microfibers a potential biomaterial for wound healing purposes.
用于伤口愈合应用的生物材料的开发需要具备多种特性，如抗菌作用、促进细胞增殖、生物相容性和生物可降解性。本研究的目的是研究玉米醇溶蛋白基微纤维的形态和力学性能，最终旨在创造一个适合伤口愈合的环境。这是通过共轴静电纺丝制备核壳微纤维来实现的，核为玉米醇溶蛋白，壳为聚环氧乙烷（PEO）。此外，将少量的PEO或硬脂酸加入到纤维核中，以改变玉米醇溶蛋白纤维的形态和力学性能。发现核中存在PEO对于形成管状纤维至关重要，而壳中的PEO增强了微纤维在水中的稳定性，并确保了微纤维垫的高弹性。盐酸四环素以无定形形式存在于纤维中，并由于纤维中形成孔隙而呈现出突释现象。所开发的系统对[具体微生物1]和[具体微生物2]具有抗菌活性，并且对成纤维细胞没有细胞毒性作用。生物相容性、抗菌活性以及良好的形态和力学性能使得所开发的玉米醇溶蛋白基微纤维成为用于伤口愈合目的的潜在生物材料。
