Division of Bioengineering, Faculty of Engineering, National University of Singapore (NUS), 7 Engineering Drive 1, Singapore 117574, Singapore.
J Control Release. 2010 Apr 19;143(2):175-82. doi: 10.1016/j.jconrel.2009.12.030. Epub 2010 Jan 11.
Hydrogel nanofiber mats based on thermoresponsive multiblock poly(ester urethane)s comprising poly(ethylene glycol) (PEG), poly(propylene glycol) (PPG), and poly(epsilon-caprolactone) (PCL) segments were fabricated by electrospinning. The hydrogel nanofiber mats were more water absorbent under cold conditions and shrunk when exposed to higher temperatures. The rate of protein release could be controlled by changing the temperature of the nanofiber environment. Cell culture studies on the nanofiber mats were carried out using human dermal fibroblasts, and healthy cell morphology was observed. The adherent viable cells were quantified by MTS after rinsing in excess buffer solution. The results showed that these nanofiber scaffolds supported excellent cell adhesion, comparable with the pure PCL nanofibers. The increased hydrophilicity of these hydrogel nanofiber mats led to a more rapid hydrolytic degradation, compared with the pure PCL nanofiber mats. These hydrogel nanofiber scaffolds could potentially be used as thermoresponsive biodegradable supporting structures for skin tissue engineering applications.
基于热响应性多嵌段聚（酯氨酯）的水凝胶纳米纤维垫由聚（乙二醇）（PEG）、聚（丙二醇）（PPG）和聚（己内酯）（PCL）段通过静电纺丝制成。水凝胶纳米纤维垫在低温下吸水性更强，暴露在较高温度下会收缩。通过改变纳米纤维环境的温度可以控制蛋白质的释放速度。用人真皮成纤维细胞进行了纳米纤维垫上的细胞培养研究，观察到健康的细胞形态。用过量缓冲溶液冲洗后，通过 MTS 定量测定贴壁活细胞。结果表明，这些纳米纤维支架支持出色的细胞黏附，与纯 PCL 纳米纤维相当。与纯 PCL 纳米纤维垫相比，这些水凝胶纳米纤维垫的亲水性增加导致更快速的水解降解。这些水凝胶纳米纤维支架可能可用作皮肤组织工程应用的热响应性可生物降解支撑结构。
