Lapolla Annunziata, Brioschi Maura, Banfi Cristina, Tremoli Elena, Bonfante Luciana, Cristoni Simone, Seraglia Roberta, Traldi Pietro
Dipartimento di Scienze Mediche e Chirurgiche, Cattedra di Malattie del Metabolismo, Università di Padova, Padova, Italy.
J Mass Spectrom. 2008 Jan;43(1):74-81. doi: 10.1002/jms.1274.
The analysis of plasma samples from healthy, diabetic and nephropathic subjects was carried out by 2D gel electrophoresis. This approach shows clear differences among the three classes of subjects. In the case of diabetic and nephropathic patients intense spots appear. Their enzymatic digestion followed by matrix assisted laser desorption ionization/mass spectrometry (MALDI/MS) analysis shows that an overexpression of unglycated and glycated ApoA-I is present in both pathological states. Interestingly, this trend is also observed for the retinol-binding protein (RBP). The data obtained can be relevant to assess possible risks associated either with the glycation level of ApoA-I or with the overexpression of RBP. In fact, in the former case possibly a different functionality of the glycated protein is to be expected, reflecting a different efficiency in cholesterol transport. In the latter case, the increase of RBP level can be related to the overweight of the diabetic subjects under investigation: it is known that obesity leads to RBP overexpression. In the case of nephropathic patients, the RBP level increases in parallel with serum creatinin.
通过二维凝胶电泳对健康、糖尿病和肾病患者的血浆样本进行了分析。这种方法显示出这三类受试者之间存在明显差异。在糖尿病和肾病患者中出现了密集的斑点。对其进行酶解，然后进行基质辅助激光解吸电离/质谱（MALDI/MS）分析，结果表明在这两种病理状态下均存在未糖基化和糖基化载脂蛋白A-I（ApoA-I）的过表达。有趣的是，视黄醇结合蛋白（RBP）也呈现出这种趋势。所获得的数据可能与评估与ApoA-I糖基化水平或RBP过表达相关的潜在风险有关。事实上，在前一种情况下，可能预期糖基化蛋白具有不同的功能，这反映了胆固醇转运效率的差异。在后一种情况下，RBP水平的升高可能与所研究的糖尿病受试者超重有关：众所周知，肥胖会导致RBP过表达。在肾病患者中，RBP水平与血清肌酐平行升高。
