CNR-ISTM, Corso Stati Uniti 4, I-35127, Padova, Italy.
J Mass Spectrom. 2013 Jan;48(1):105-10. doi: 10.1002/jms.3113.
Increased oxidation of low density lipoprotein (LDL) is characteristic of atherosclerosis. In this frame, high density lipoproteins (HDL) play an important role, being able to remove lipid peroxides (LPOs) and cholesterol from oxidized LDL, so exhibiting a protective role against atherosclerosis. A wide range of reactive compounds lead to the oxidation of methionine (Met) residues with the formation of methionine sulphoxide (MetO) in apolipoprotein A-I (ApoA-I). Consequently, the determination of MetO level can give both an evaluation of oxidative stress and the reduced capability of ApoA-I in LPOs and cholesterol transport. For these reasons, the development of analytical methods able to determine the MetO level is surely of interest, and we report here the results obtained by MALDI mass spectrometry.
低密度脂蛋白（LDL）的氧化增加是动脉粥样硬化的特征。在这种情况下，高密度脂蛋白（HDL）起着重要的作用，能够从氧化的 LDL 中去除脂质过氧化物（LPO）和胆固醇，因此对动脉粥样硬化具有保护作用。大量的反应性化合物导致载脂蛋白 A-I（ApoA-I）中蛋氨酸（Met）残基的氧化，形成蛋氨酸亚砜（MetO）。因此，MetO 水平的测定既能评估氧化应激，又能评估 ApoA-I 在 LPO 和胆固醇转运中的能力降低。基于这些原因，开发能够测定 MetO 水平的分析方法无疑是很有意义的，我们在这里报告了 MALDI 质谱法得到的结果。
