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坦桑尼亚维多利亚湖地区医疗机构艾滋病毒检测质量保证评估

Assessment of quality assurance in HIV testing in health facilities in Lake Victoria zone, Tanzania.

作者信息

Mashauri F M, Siza J E, Temu M M, Mngara J T, Kishamawe C, Changalucha J M

机构信息

National Institute for Medical Research, Mwanza Research Centre, P.O Box 14 62, Mwanza, Tanzania.

出版信息

Tanzan Health Res Bull. 2007 May;9(2):110-4. doi: 10.4314/thrb.v9i2.14312.

Abstract

Tanzania is currently implementing the antiretroviral treatment programme, and has a target of putting about 400,000 eligible HIV infected individuals on treatment by 2008. This will involve screening a large number of people, which will require non-laboratory personnel to be involved in doing HIV testing. In order to guarantee reliable and quality HIV test results, there is a need to ensure that quality assurance (QA) procedures are followed from specimen collection, testing and reporting of results. In light of the above a survey was conducted to assess QA in HIV testing in health facilities in Lake Victoria zone, Tanzania. A total of 89 health facilities (29 hospitals, 34 health centres, 9 dispensaries and 17 voluntary and counselling testing centres) were surveyed. Only three (10.3%) health facilities reported performing Uniform II ELISA for HIV diagnosis. All other health facilities reported to be using HIV rapid tests Capillus and Determine. Five (5.6%) of health facility laboratories performed CD4 counts. Internal quality control (IQC) were performed in 21 (63.6%) of the hospitals. Kits for HIV testing were reported to be readily available by 54 (60.7%) of the facilities. Only 16 (18%) of the health facilities had standard operating procedures in place. Systems of equipment calibration were reported by 13 (14.6%) of the health facilities. Counselling services were available in all health facilities and all counsellors had received the 6-week mandatory training course. These findings show that most of health facilities in the Lake Victoria zone do not adhere to QA procedures in HIV testing. There is therefore, a need to establish a monitoring system to laboratories performing HIV testing for the purpose of ensuring QA procedures are done. Personnel doing HIV testing should be re-trained at a regular basis to cope with new techniques and ensure QA procedures are followed.

摘要

坦桑尼亚目前正在实施抗逆转录病毒治疗项目,目标是到2008年使约40万符合条件的艾滋病毒感染者接受治疗。这将涉及对大量人群进行筛查,这就需要非实验室人员参与艾滋病毒检测工作。为了确保艾滋病毒检测结果可靠且质量达标,有必要确保从样本采集、检测到结果报告都遵循质量保证(QA)程序。鉴于上述情况,开展了一项调查以评估坦桑尼亚维多利亚湖地区医疗机构艾滋病毒检测中的质量保证情况。共对89家医疗机构(29家医院、34家保健中心、9家诊疗所和17家自愿咨询检测中心)进行了调查。只有三家(10.3%)医疗机构报告使用统一II型酶联免疫吸附测定法进行艾滋病毒诊断。所有其他医疗机构报告称使用艾滋病毒快速检测法“卡皮勒斯”和“确定”。5家(5.6%)医疗机构实验室进行了CD4细胞计数。21家(63.6%)医院进行了内部质量控制。54家(60.7%)机构报告称艾滋病毒检测试剂盒随时可用。只有16家(18%)医疗机构制定了标准操作程序。13家(14.6%)医疗机构报告有设备校准系统。所有医疗机构都提供咨询服务,且所有咨询师都接受了为期6周的强制培训课程。这些调查结果表明,维多利亚湖地区的大多数医疗机构在艾滋病毒检测中未遵循质量保证程序。因此,有必要为进行艾滋病毒检测的实验室建立一个监测系统,以确保质量保证程序得到执行。从事艾滋病毒检测的人员应定期接受再培训,以掌握新技术并确保遵循质量保证程序。

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