用丙烯酰胺作为载体，利用固定化肠膜明串珠菌 KIBGE HA1 细胞连续生产右旋糖酐。
Continuous Production of Dextran from Immobilized Cells of Leuconostoc mesenteroides KIBGE HA1 Using Acrylamide as a Support.
机构信息
The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270 Pakistan.
出版信息
Indian J Microbiol. 2011 Jul;51(3):279-82. doi: 10.1007/s12088-011-0130-5. Epub 2011 Jan 26.
The cells of L. mesenteroides KIBGE HA1 were immobilized for the production of dextran on acrylamide gel and gel concentration was optimized for maximum entrapment. Sucrose at substrate concentration of 10% produced high yield of dextran at 25°C with a percent conversion of 5.82 while at 35°C it was 3.5. However, increasing levels of sucrose diminished dextran yields. The free cells stopped producing dextran after 144 h, while immobilized cells continued to produce dextran even after 480 h. Molecular mass distribution of dextran from free cells indicate that it is identical to that of blue dextran while the molecular mass of dextran from immobilized cells is lower than that of free cells.
肠膜明串珠菌 KIBGE HA1 细胞被固定化以在丙烯酰胺凝胶上生产右旋糖酐，并优化凝胶浓度以实现最大包埋。在 25°C 下，当底物浓度为 10%的蔗糖产生了高产量的右旋糖酐，转化率为 5.82%，而在 35°C 时为 3.5%。然而，蔗糖浓度的增加会降低右旋糖酐的产量。游离细胞在 144 小时后停止生产右旋糖酐，而固定化细胞甚至在 480 小时后仍继续生产右旋糖酐。游离细胞产生的右旋糖酐的分子量分布表明它与蓝色右旋糖酐相同，而固定化细胞产生的右旋糖酐的分子量低于游离细胞。
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