Ohlsson K, Skude G
Clin Chim Acta. 1976 Jan 2;66(1):1-7. doi: 10.1016/0009-8981(76)90365-x.
Complex formation in vitro between human alpha2-macroglobulin and the human proteases cationic trypsin, chymotrypsin, plasmin and granulocyte elastase and collagenase was clearly visualized by the use of thin-layer electrofocusing in polyacrylamide gel followed by electrophoresis in agarose gel containing antibodies against human alpha2-macroglobulin. The technique permits semi-quantitative determination of the amount of complex and can demonstrate the formation of complexes between alpha2-macroglobulin and protease in vivo in ascitic fluid in peritonitis.
通过在聚丙烯酰胺凝胶中进行薄层电聚焦，随后在含有抗人α2-巨球蛋白抗体的琼脂糖凝胶中进行电泳，清晰地观察到了人α2-巨球蛋白与人类蛋白酶阳离子胰蛋白酶、胰凝乳蛋白酶、纤溶酶、粒细胞弹性蛋白酶和胶原酶在体外形成的复合物。该技术可对复合物的量进行半定量测定，并且能够证明α2-巨球蛋白与蛋白酶在体内腹膜炎腹水形成的复合物。
