Bohe M, Borgström A, Lindström C, Ohlsson K
J Clin Pathol. 1986 Jul;39(7):786-93. doi: 10.1136/jcp.39.7.786.
Normal and metaplastic gastrointestinal mucosa obtained at surgical resection were studied by light microscopy, using the unlabelled antibody enzyme method for immunohistochemical staining of lysozyme, pancreatic endoproteases, and pancreatic secretory trypsin inhibitor (PSTI). Paneth cells in the mucosa of normal small intestine, gastric mucosa with intestinal metaplasia, and colonic metaplastic mucosa were found to contain anionic trypsin, cationic trypsin, lysozyme, and PSTI immunoreactivity, but not chymotrypsin and elastase immunoreactivity. Normal gastric and colonic mucosa and some goblet cells in the small intestine showed positive PSTI immunoreactivity but no endoprotease immunoreactivity. The presence of immunoreactive trypsin and immunoreactive PSTI in the Paneth cells, which are of secretory type, probably indicates an important extrapancreatic source of these proteins rather than a storage of endocytosed material.
采用未标记抗体酶法对溶菌酶、胰腺内蛋白酶和胰腺分泌性胰蛋白酶抑制剂（PSTI）进行免疫组织化学染色，通过光学显微镜对手术切除获取的正常和化生的胃肠道黏膜进行研究。结果发现，正常小肠黏膜、肠化生的胃黏膜和结肠化生黏膜中的潘氏细胞含有阴离子胰蛋白酶、阳离子胰蛋白酶、溶菌酶和PSTI免疫反应性，但不含有糜蛋白酶和弹性蛋白酶免疫反应性。正常胃和结肠黏膜以及小肠中的一些杯状细胞显示PSTI免疫反应性阳性，但无内蛋白酶免疫反应性。分泌型的潘氏细胞中存在免疫反应性胰蛋白酶和免疫反应性PSTI，这可能表明这些蛋白质的一个重要的胰腺外来源，而非内吞物质的储存。
