Wang Xiao-Ping, Lin Li, Chen Zhen-Lin, Ma Yan-Yan, Pan Jian-Guo
College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou 510405, China.
Zhong Yao Cai. 2007 May;30(5):560-2.
To establish an HPLC method for determination of galangin in propolis.
HPLC method was established. Diamonsil C18 column was used, CH3OH-4% H3PO4 solution (65:35) as mobile phase, at the flow rate of 1.0 ml/min and the UV detection wavelength at 256 nm.
The linear of galangin was 0.00412 - 0.02472 mg/ml (r2 = 0. 9998). The content of galangin was the highest in the propolis from Shandong (11.7 mg/g). The content of galangin was higher in the propolis from Neimeng and Henan, respectively 10. 5 mg/g,10. 3 mg/g, the content of galangin was the lowest in the propolis from Gansu (9.8 mg/g).
HPLC method is simple and reliable for determination of galangin in propolis.
建立蜂胶中高良姜素的高效液相色谱测定方法。
建立高效液相色谱法。采用 Diamonsil C18 柱，以甲醇 - 4%磷酸溶液（65:35）为流动相，流速为 1.0 ml/min，紫外检测波长为 256 nm。
高良姜素的线性范围为 0.00412 - 0.02472 mg/ml（r2 = 0.9998）。山东蜂胶中高良姜素含量最高（11.7 mg/g）。内蒙古和河南蜂胶中高良姜素含量较高，分别为 10.5 mg/g、10.3 mg/g，甘肃蜂胶中高良姜素含量最低（9.8 mg/g）。
高效液相色谱法测定蜂胶中高良姜素简单可靠。
