Lu Jin-qing, Chen Li, Xiao Bo, Wan Wei, Yu Fan, Tian Yao-ping
Hubei College of Traditional Chinese Medicine, Wuhan 430061, China.
Zhong Yao Cai. 2007 Aug;30(8):929-32.
To establish the HPLC fingerprint spectrum of flavonoids from Gynostemma pentaphyllum.
The HPLC conditions with TURNER Kromasil C18 column (250 x 4. 6 mm, 5 microm) was used, the mobile phase consisted of 0.4% phosphoric acid-methanol-acetonitrile with gradient elution at flow rate of 0.5 ml/min and the detection wavelength was set at 360nm.
Ten common characteristic peaks were taken as fingerprint peaks the presision and the retest are in accordance with the requirement.
The method is stable, reliable, precision and provides a scientific basis for the quality standard of Gynostemma pentaphyllum.
建立绞股蓝黄酮类成分的高效液相色谱指纹图谱。
采用TURNER Kromasil C18柱（250×4.6mm，5μm），以0.4%磷酸-甲醇-乙腈为流动相进行梯度洗脱，流速为0.5ml/min，检测波长为360nm。
确定10个共有特征峰作为指纹图谱峰，精密度和重复性均符合要求。
该方法稳定、可靠、精密，为绞股蓝质量标准的制定提供了科学依据。
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