Majumder Avishek, Goyal Arun
Department of Biotechnology, Indian Institute of Technology Guwahati, North Guwahati, Guwahati 781 039, Assam, India.
Bioresour Technol. 2008 Jun;99(9):3685-91. doi: 10.1016/j.biortech.2007.07.027. Epub 2007 Aug 28.
Statistically-based experimental designs were applied to optimize the fermentation for the production of glucosyltransferase by Leuconostoc dextranicum NRRL B-1146. Eleven medium components were examined for their significance on enzyme production using Plackett-Burman factorial design. Tween 80, sucrose and K2HPO4 significantly improved the enzyme production process. The combined effect of these nutrients on glucansucrase production were studied using a 2 2 full-factorial central composite design, a second-order polynomial was established to identify the relationship between the enzyme output and the three medium components. The optimal concentration of variables for maximum glucansucrase production were Tween 80 (0.55%, v/v); sucrose (5.6%, w/v) and K2HPO4 (1%, w/v). The maximum enzyme activity by predicted model was 6.53 U/ml that was in perfect agreement with the actual experimental value (6.40 U/ml).
基于统计学的实验设计被用于优化右旋糖明串珠菌NRRL B - 1146生产葡糖基转移酶的发酵过程。使用Plackett - Burman析因设计考察了11种培养基成分对酶产量的重要性。吐温80、蔗糖和磷酸氢二钾显著改善了酶的生产过程。使用2²全因子中心复合设计研究了这些营养物质对葡聚糖蔗糖酶生产的综合影响，建立了二阶多项式以确定酶产量与三种培养基成分之间的关系。葡聚糖蔗糖酶产量最高时各变量的最佳浓度为：吐温80（0.55%，v/v）；蔗糖（5.6%，w/v）和磷酸氢二钾（1%，w/v）。预测模型得出的最大酶活性为6.53 U/ml，与实际实验值（6.40 U/ml）完全一致。
