一种用于估算N-乙酰-D-葡萄糖胺的比色法的非特异性
Non-specificity of a colorimetric method for the estimation of N-acetyl-D-glucosamine.
作者信息
Domard A, Vasseur V
机构信息
Laboratoire d'Etude des Matériaux Plastiques et des Biomatériaux (URA CNRS 507), Université Claude Bernard, Villeubanne, France.
出版信息
Int J Biol Macromol. 1991 Dec;13(6):366-8. doi: 10.1016/0141-8130(91)90020-u.
The colorimetric method of Reissig et al. for the estimation of N-acetylamino sugars, is often used as a specific method for the quantification of the N-acetyl-D-glucosamine. Although this assay is more sensitive to the monomer, it recognizes all soluble N-acetyl-D-glucosamine oligomers. This result is very important because this method is extensively used in biology for the estimation of chitinolytic activity.
赖西格等人用于估算N - 乙酰氨基糖的比色法，常被用作定量N - 乙酰 - D - 葡萄糖胺的特定方法。尽管该测定法对单体更敏感，但它能识别所有可溶性N - 乙酰 - D - 葡萄糖胺寡聚物。这一结果非常重要，因为该方法在生物学中被广泛用于估算几丁质分解活性。
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