Science. 1972 Jan 28;175(4020):451-4. doi: 10.1126/science.175.4020.451.
A tactile stimulus to the siphon of Aplysia produces a defensive withdrawal reflex consisting of contraction of the siphon, the gill, and the mantle shelf. We studied long-term habituation of this reflex using two types of preparations, one focusing on the siphon component and the other on the gill component of the reflex. Siphon withdrawal, studied in unrestrained animals, showed marked habituation within a single ten-trial training session. Five daily training sessions produced habituaton that built up across days and lasted for at least 3 weeks. Furthermore, spaced training produced significantly longer lasting habituation than massed training. Gill withdrawal, studied in a restrained animal, also showed long-term retention of habituation. Since the neural circuitry of gill withdrawal is relatively well understood, it may be possible to study the cellular mechanisms underlying a long-term behavioral modification.
当用触须刺激海兔的虹吸管时，会产生一个防御性的退缩反射，包括虹吸管、腮和套膜的收缩。我们使用两种类型的制剂研究了这种反射的长期习惯化，一种制剂侧重于反射的虹吸管成分，另一种制剂侧重于腮成分。在未受约束的动物中研究虹吸管的退缩反应，在单个十次训练中就表现出明显的习惯化。五次每日训练产生了在数天内逐渐建立并持续至少 3 周的习惯化。此外，间隔训练产生的习惯化持续时间明显长于集中训练。在受约束的动物中研究腮的退缩反应，也表现出长期保留的习惯化。由于腮退缩的神经回路相对较好理解，因此可能有可能研究长期行为改变的细胞机制。
