鸟类红细胞组蛋白H1互补物的比较。

A comparison of the histone H1 complements of avian erythrocytes.

Pałyga J

Department of Genetics, Educational University of Kielce, Poland.

Int J Biochem. 1991;23(9):845-9. doi: 10.1016/0020-711x(91)90069-y.

Abstract

Histone H1 from chicken, turkey, duck and goose erythrocytes was resolved into six bands and that from quail into seven bands in an acetic acid-urea polyacrylamide gel. 2. A fast migrating minor subtype H1.e was detected in avian erythrocytes using two-dimensional polyacrylamide gel electrophoresis. 3. Although histone subtype H1.z from quail, turkey and duck was well separated in acid-urea gel, a similar protein in goose was found only in two-dimensional gel. This spot was absent in chicken. 4. Histone H1 spots .c, .c' and .d migrate in two-dimensional gel in a relatively constant manner forming a triangle-shaped pattern that facilitates comparison of H1 subtypes among various avian species.

摘要

在乙酸 - 尿素聚丙烯酰胺凝胶中，鸡、火鸡、鸭和鹅红细胞中的组蛋白H1可分离为六条带，鹌鹑红细胞中的组蛋白H1可分离为七条带。2. 使用二维聚丙烯酰胺凝胶电泳在鸟类红细胞中检测到一种快速迁移的次要亚型H1.e。3. 虽然鹌鹑、火鸡和鸭的组蛋白亚型H1.z在酸 - 尿素凝胶中能很好地分离，但鹅中的类似蛋白仅在二维凝胶中被发现。鸡中不存在这个斑点。4. 组蛋白H1斑点.c、.c' 和.d在二维凝胶中以相对恒定的方式迁移，形成三角形图案，便于比较不同鸟类物种之间的H1亚型。