某些禽类胆汁免疫球蛋白结构的比较研究。I. 鸡、火鸡、鸭和鹅胆汁免疫球蛋白的物理化学性质。

Comparative studies on the structure of biliary immunoglobulins of some avian species. I. Physico-chemical properties of biliary immunoglobulins of chicken, turkey, duck and goose.

作者信息

Hädge D, Ambrosius H

机构信息

Laboratory of Immunobiology, Karl Marx University, Leipzig, G.D.R.

出版信息

Dev Comp Immunol. 1988 Winter;12(1):121-9. doi: 10.1016/0145-305x(88)90030-4.

DOI:10.1016/0145-305x(88)90030-4

PMID:3350185

Abstract

In pooled bile of chicken, turkey, duck and goose immunoglobulins (Igs)+ were found in relatively high amounts between 4.5 and 15.0 mg/ml (corresponding to 28 to 36% of the total protein contents). All biliary Ig fractions studied possess (H2L2)n structure estimated by SDS-PAGE. In chicken and turkey L chains are assumed to be non-covalently linked to the polymeric H chains. In duck and goose L chains are completely linked to H chains. The Mr of H chains are rather similar: 64 kDa for the Igs of chicken and turkey bile and 67 kDa for the biliary Igs of duck and goose. There are slight differences in the electrophoretic mobility: Chicken and turkey Igs are beta 1/alpha 2-globulins and the corresponding Ig fractions from duck and goose are beta 2-globulins.

摘要

在鸡、火鸡、鸭和鹅的混合胆汁中，发现免疫球蛋白（Igs）+ 的含量相对较高，在4.5至15.0毫克/毫升之间（相当于总蛋白质含量的28%至36%）。通过SDS-PAGE估计，所有研究的胆汁Ig组分均具有（H2L2）n结构。在鸡和火鸡中，轻链被认为与聚合重链非共价连接。在鸭和鹅中，轻链与重链完全连接。重链的相对分子质量相当相似：鸡和火鸡胆汁中的Igs为64 kDa，鸭和鹅的胆汁Igs为67 kDa。电泳迁移率存在细微差异：鸡和火鸡的Igs是β1/α2球蛋白，鸭和鹅相应的Ig组分是β2球蛋白。