Pałyga J, Górnicka-Michalska E, Kowalski A
Department of Genetics, Educational University of Kielce, Warszawska, Poland.
Biochem J. 1993 Sep 15;294 ( Pt 3)(Pt 3):859-63. doi: 10.1042/bj2940859.
Three different phenotypes of erythrocyte histone H1.z were detected in several lines of duck by using two-dimensional PAGE. Electrophoresis and inheritance data have shown that two co-dominant alleles, z1 and z2, encode two proteins which differ slightly in their apparent molecular masses. Allele z1 was very abundant and was found at a frequency at least 0.94, while allele z2 was very rare and was present at a frequency of less than 0.06. Limited chemical and enzymic cleavages appeared to indicate that the allelic forms of histone H1.z differed in the C-terminal regions of their molecules.
通过二维聚丙烯酰胺凝胶电泳(2D-PAGE)在多个鸭品系中检测到了三种不同表型的红细胞组蛋白H1.z。电泳和遗传数据表明,两个共显性等位基因z1和z2编码两种表观分子量略有差异的蛋白质。等位基因z1非常丰富,其出现频率至少为0.94,而等位基因z2非常罕见,出现频率小于0.06。有限的化学和酶切分析似乎表明,组蛋白H1.z的等位基因形式在其分子的C末端区域存在差异。