通过高效液相色谱法鉴定分枝杆菌。
Identification of mycobacteria by high-performance liquid chromatography.
作者信息
Butler W R, Jost K C, Kilburn J O
机构信息
Division of Bacterial and Mycotic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
出版信息
J Clin Microbiol. 1991 Nov;29(11):2468-72. doi: 10.1128/jcm.29.11.2468-2472.1991.
Abstract
Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described.
摘要
通过高效液相色谱法（HPLC）对从皂化分枝杆菌细胞中提取的霉菌酸进行对溴苯甲酰酯检测。通过检测来自培养物保藏中心的菌株和其他特征明确的分离株，建立了分枝杆菌属各菌种的标准HPLC图谱。利用峰的相对保留时间和峰高比较，制定了一种鉴别方案，对所检测的菌种鉴别准确率达98%。本文描述了一种快速、经济高效的HPLC方法，该方法为分枝杆菌的鉴定提供了另一种途径。
相似文献
1
Identification of mycobacteria by high-performance liquid chromatography.
J Clin Microbiol. 1991 Nov;29(11):2468-72. doi: 10.1128/jcm.29.11.2468-2472.1991.
2
Characterization of mycobacteria species by HPLC and pattern recognition.
J Chromatogr Sci. 1994 Jun;32(6):219-27. doi: 10.1093/chromsci/32.6.219.
3
[Identification of Mycobacterium species using reversed-phase high performance liquid chromatographic analysis of mycolic acid].
Se Pu. 2008 Sep;26(5):534-9. doi: 10.1016/s1872-2059(08)60027-9.
4
Rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a Mycobacterium library.
J Clin Microbiol. 1994 Mar;32(3):740-5. doi: 10.1128/jcm.32.3.740-745.1994.
5
Routine application of high-performance liquid chromatography for identification of mycobacteria.
J Clin Microbiol. 1993 Jul;31(7):1759-63. doi: 10.1128/jcm.31.7.1759-1763.1993.
6
Application of the Sherlock Mycobacteria Identification System using high-performance liquid chromatography in a clinical laboratory.
J Clin Microbiol. 2001 Mar;39(3):964-70. doi: 10.1128/JCM.39.3.964-970.2001.
7
[Mycolic acids analysis from various species mycobacterium by high pressure liquid chromatography (HPLC)].
Pneumonol Alergol Pol. 2002;70(3-4):130-8.
8
Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids.
J Clin Microbiol. 1988 Jan;26(1):50-3. doi: 10.1128/jcm.26.1.50-53.1988.
9
High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.
J Clin Microbiol. 1990 Sep;28(9):2094-8. doi: 10.1128/jcm.28.9.2094-2098.1990.
10
A simple chemical test to distinguish mycobacteria from other mycolic-acid-containing actinomycetes.
J Gen Microbiol. 1993 Sep;139(9):2203-13. doi: 10.1099/00221287-139-9-2203.
引用本文的文献
1
Non-Microbiological Mycobacterial Detection Techniques for Quality Control of Biological Products: A Comprehensive Review.
Microorganisms. 2024 Apr 12;12(4):788. doi: 10.3390/microorganisms12040788.
2
Ocular infections associated with atypical mycobacteria: A review.
Indian J Ophthalmol. 2024 Jan 1;72(1):19-28. doi: 10.4103/IJO.IJO_560_23. Epub 2023 Dec 22.
3
Methods of Identifying Strains in Clinical Samples.
Pathogens. 2022 Dec 8;11(12):1496. doi: 10.3390/pathogens11121496.
4
Comparative analysis of volatile organic compounds for the classification and identification of mycobacterial species.
PLoS One. 2018 Mar 20;13(3):e0194348. doi: 10.1371/journal.pone.0194348. eCollection 2018.
5
The Use of High Performance Liquid Chromatography to Speciate and Characterize the Epidemiology of Mycobacteria.
Lab Med. 2011 Oct 1;42(10):612-617. doi: 10.1309/LMDDEHPSYE6ZDM3C.
6
A simple and efficient multiplex PCR assay for the identification of Mycobacterium genus and Mycobacterium tuberculosis complex to the species level.
Yonsei Med J. 2013 Sep;54(5):1220-6. doi: 10.3349/ymj.2013.54.5.1220.
7
Validation of Mycobacterium tuberculosis Rv1681 protein as a diagnostic marker of active pulmonary tuberculosis.
J Clin Microbiol. 2013 May;51(5):1367-73. doi: 10.1128/JCM.03192-12. Epub 2013 Feb 6.
8
Polymerase chain reaction in the diagnosis of tuberculosis.
Indian J Clin Biochem. 2000 Aug;15(Suppl 1):200-16. doi: 10.1007/BF02867559.
9
Rapid DNA extraction for specific detection and quantitation of Mycobacterium tuberculosis DNA in sputum specimens using Taqman assays.
Tuberculosis (Edinb). 2011 Dec;91 Suppl 1:S43-8. doi: 10.1016/j.tube.2011.10.009. Epub 2011 Nov 15.
10
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of mycobacteria in routine clinical practice.
PLoS One. 2011;6(9):e24720. doi: 10.1371/journal.pone.0024720. Epub 2011 Sep 13.
本文引用的文献
1
Lipid composition in the classification and identification of acid-fast bacteria.
Soc Appl Bacteriol Symp Ser. 1980;8:189-256.
2
Mycolic acid patterns of some species of Mycobacterium.
Arch Microbiol. 1984 Oct;139(2-3):225-31. doi: 10.1007/BF00402005.
3
Effect of isoniazid on the in vivo mycolic acid synthesis, cell growth, and viability of Mycobacterium tuberculosis.
Antimicrob Agents Chemother. 1972 Jul;2(1):29-35. doi: 10.1128/AAC.2.1.29.
4
Determination of molecular species composition of C80 or longer-chain alpha-mycolic acids in Mycobacterium spp. by gas chromatography-mass spectrometry and mass chromatography.
J Clin Microbiol. 1986 Dec;24(6):1060-70. doi: 10.1128/jcm.24.6.1060-1070.1986.
5
Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids.
J Clin Microbiol. 1988 Jan;26(1):50-3. doi: 10.1128/jcm.26.1.50-53.1988.
6
Gen-probe test should not be considered final in Mycobacterium tuberculosis identification.
J Clin Microbiol. 1989 Jan;27(1):229. doi: 10.1128/jcm.27.1.229-.1989.
7
Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium.
Int J Syst Bacteriol. 1990 Oct;40(4):323-30. doi: 10.1099/00207713-40-4-323.
8
High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.
J Clin Microbiol. 1990 Sep;28(9):2094-8. doi: 10.1128/jcm.28.9.2094-2098.1990.
9
Phenacyl esters of fatty acids via crown ether catalysts for enhanced ultraviolet detection in liquid chromatography.
Anal Chem. 1975 Sep;47(11):1797-1801. doi: 10.1021/ac60361a025.
10
Separation of the mixture of trehalose 6,6'-dimycolates comprising the mycobacterial glycolipid fraction, "P3".
Biochem Biophys Res Commun. 1977 Jul 25;77(2):449-56. doi: 10.1016/s0006-291x(77)80001-6.
Zotero 插件