高效液相色谱法在分枝杆菌鉴定中的常规应用。
Routine application of high-performance liquid chromatography for identification of mycobacteria.
作者信息
Thibert L, Lapierre S
机构信息
Laboratoire de Santé Publique du Québec, Sainte-Anne-de-Vellevue, Canada.
出版信息
J Clin Microbiol. 1993 Jul;31(7):1759-63. doi: 10.1128/jcm.31.7.1759-1763.1993.
Abstract
Mycolic acid analysis by high-performance liquid chromatography (HPLC) was introduced in our laboratory as the routine technique for identifying all clinical isolates of mycobacteria referred to us. HPLC identified 96.1% of the 1,103 strains analyzed, whereas the biochemical procedures and/or the commercial DNA probes identified 98.3% of strains, for an overall agreement of 94.4%. Compared with the probes, there was 100% specificity and 98.9% sensitivity for Mycobacterium tuberculosis identification. HPLC allowed early detection and identification of the rare mycobacterial species M. haemophilum, M. malmoense, M. shimoidei, and M. fallax as well as uncharacteristic strains of M. simiae. After 18 months of routine use, HPLC proved to be reliable, easy to perform, rapid, and less costly than other identification methods.
摘要
我们实验室采用高效液相色谱法（HPLC）对分枝杆菌临床分离株进行菌酸分析，作为鉴定所有送检临床分离株的常规技术。HPLC对1103株分析菌株中的96.1%进行了鉴定，而生化方法和/或商业DNA探针鉴定出98.3%的菌株，总体一致性为94.4%。与探针相比，结核分枝杆菌鉴定的特异性为100%，敏感性为98.9%。HPLC能够早期检测和鉴定罕见分枝杆菌菌种嗜血性分枝杆菌、马尔默分枝杆菌、下田分枝杆菌和类产碱假单胞菌以及不典型的猿分枝杆菌菌株。经过18个月的常规使用，HPLC被证明是可靠的，操作简便、快速，且比其他鉴定方法成本更低。
相似文献
1
Routine application of high-performance liquid chromatography for identification of mycobacteria.高效液相色谱法在分枝杆菌鉴定中的常规应用。
J Clin Microbiol. 1993 Jul;31(7):1759-63. doi: 10.1128/jcm.31.7.1759-1763.1993.
2
Rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a Mycobacterium library.使用模式识别软件和分枝杆菌文库，通过高效液相色谱法快速鉴定分枝杆菌的分枝菌酸模式。
J Clin Microbiol. 1994 Mar;32(3):740-5. doi: 10.1128/jcm.32.3.740-745.1994.
3
Curvilinear-gradient high-performance liquid chromatography for identification of mycobacteria.用于分枝杆菌鉴定的曲线梯度高效液相色谱法
J Clin Microbiol. 1993 Jul;31(7):1876-81. doi: 10.1128/jcm.31.7.1876-1881.1993.
4
Application of the Sherlock Mycobacteria Identification System using high-performance liquid chromatography in a clinical laboratory.在临床实验室中使用高效液相色谱法的Sherlock分枝杆菌鉴定系统的应用。
J Clin Microbiol. 2001 Mar;39(3):964-70. doi: 10.1128/JCM.39.3.964-970.2001.
5
Utility of high-performance liquid chromatography analysis of mycolic acids and partial 16S rRNA gene sequencing for routine identification of Mycobacterium spp. in a national reference laboratory.高效液相色谱法分析分枝菌酸和部分 16S rRNA 基因测序在国家参考实验室常规鉴定分枝杆菌属的应用。
Diagn Microbiol Infect Dis. 2010 Jun;67(2):143-52. doi: 10.1016/j.diagmicrobio.2010.02.011.
6
Identification of mycobacteria by high-performance liquid chromatography.通过高效液相色谱法鉴定分枝杆菌。
J Clin Microbiol. 1991 Nov;29(11):2468-72. doi: 10.1128/jcm.29.11.2468-2472.1991.
7
A comparison of mycolic acid analysis for nontuberculous mycobacteria identification by thin-layer chromatography and molecular methods.通过薄层色谱法和分子方法对非结核分枝杆菌进行鉴定的分枝菌酸分析比较
Microbiol Immunol. 2005;49(7):571-8. doi: 10.1111/j.1348-0421.2005.tb03642.x.
8
Comparison among three methods for mycobacteria identification.三种分枝杆菌鉴定方法的比较。
Salud Publica Mex. 2000 Nov-Dec;42(6):484-9. doi: 10.1590/s0036-36342000000600003.
9
Direct identification of Mycobacterium species in BACTEC 7H12B medium by high-performance liquid chromatography.通过高效液相色谱法直接鉴定BACTEC 7H12B培养基中的分枝杆菌种类。
J Clin Microbiol. 1994 Feb;32(2):521-4. doi: 10.1128/jcm.32.2.521-524.1994.
10
Identification of mycobacteria by thin layer chromatographic analysis of mycolic acids and conventional biochemical method: four years of experience.通过分枝菌酸的薄层色谱分析和传统生化方法鉴定分枝杆菌：四年经验
Mem Inst Oswaldo Cruz. 1998 Nov-Dec;93(6):801-5. doi: 10.1590/s0074-02761998000600019.
引用本文的文献
1
atpE gene as a new useful specific molecular target to quantify Mycobacterium in environmental samples.atpE 基因作为一种新的有用的特定分子靶标，可用于定量环境样本中的分枝杆菌。
BMC Microbiol. 2013 Dec 3;13:277. doi: 10.1186/1471-2180-13-277.
2
Clinical manifestations, diagnosis, and treatment of Mycobacterium haemophilum infections.嗜血分枝杆菌感染的临床表现、诊断与治疗。
Clin Microbiol Rev. 2011 Oct;24(4):701-17. doi: 10.1128/CMR.00020-11.
3
Current issues on molecular and immunological diagnosis of tuberculosis.结核病分子与免疫诊断的当前问题
Yonsei Med J. 2007 Jun 30;48(3):347-59. doi: 10.3349/ymj.2007.48.3.347.
4
Comparison of three methods for rapid identification of mycobacterial clinical isolates to the species level.三种将分枝杆菌临床分离株快速鉴定到种水平方法的比较。
J Clin Microbiol. 2007 Jun;45(6):1898-903. doi: 10.1128/JCM.02253-06. Epub 2007 Mar 14.
5
Characterization of a novel group of mycobacteria and proposal of Mycobacterium sherrisii sp. nov.一组新型分枝杆菌的鉴定及新种谢里斯分枝杆菌的提议
J Clin Microbiol. 2004 Jan;42(1):52-9. doi: 10.1128/JCM.42.1.52-59.2004.
6
Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria.致病性非色素沉着或迟色素沉着快速生长分枝杆菌的临床和分类学地位
Clin Microbiol Rev. 2002 Oct;15(4):716-46. doi: 10.1128/CMR.15.4.716-746.2002.
7
Identification of Mycobacterium spp. by using a commercial 16S ribosomal DNA sequencing kit and additional sequencing libraries.使用商业16S核糖体DNA测序试剂盒和额外的测序文库鉴定分枝杆菌属。
J Clin Microbiol. 2002 Feb;40(2):400-6. doi: 10.1128/JCM.40.2.400-406.2002.
8
Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species.通过高效液相色谱法进行分枝菌酸分析以鉴定分枝杆菌菌种。
Clin Microbiol Rev. 2001 Oct;14(4):704-26, table of contents. doi: 10.1128/CMR.14.4.704-726.2001.
9
Application of the Sherlock Mycobacteria Identification System using high-performance liquid chromatography in a clinical laboratory.在临床实验室中使用高效液相色谱法的Sherlock分枝杆菌鉴定系统的应用。
J Clin Microbiol. 2001 Mar;39(3):964-70. doi: 10.1128/JCM.39.3.964-970.2001.
10
Advances in diagnosis of tuberculosis.结核病诊断的进展
Indian J Pediatr. 1996 Mar-Apr;63(2):153-7. doi: 10.1007/BF02845238.
本文引用的文献
1
Identification of clinically significant Mycobacterium fortuitum complex isolates.具有临床意义的偶然分枝杆菌复合群菌株的鉴定。
J Clin Microbiol. 1981 Dec;14(6):686-91. doi: 10.1128/jcm.14.6.686-691.1981.
2
[Taxonomic value of mycobacterial fatty acids: proposal for a method of analysis].[分枝杆菌脂肪酸的分类学价值：一种分析方法的建议]
Ann Microbiol (Paris). 1983 Sep-Oct;134B(2):241-56.
3
Mycolic acid analysis for clinical identification of Mycobacterium avium and related mycobacteria.用于鸟分枝杆菌及相关分枝杆菌临床鉴定的分枝菌酸分析
J Clin Microbiol. 1986 Nov;24(5):835-9. doi: 10.1128/jcm.24.5.835-839.1986.
4
High-performance liquid chromatography of mycolic acids as a tool in the identification of Corynebacterium, Nocardia, Rhodococcus, and Mycobacterium species.分枝菌酸的高效液相色谱法作为鉴定棒状杆菌属、诺卡氏菌属、红球菌属和分枝杆菌属菌种的工具。
J Clin Microbiol. 1986 Jan;23(1):182-5. doi: 10.1128/jcm.23.1.182-185.1986.
5
High-performance liquid chromatography analysis of mycolic acids as an aid in laboratory identification of Rhodococcus and Nocardia species.分枝菌酸的高效液相色谱分析辅助红球菌属和诺卡菌属菌种的实验室鉴定
J Clin Microbiol. 1987 Nov;25(11):2126-31. doi: 10.1128/jcm.25.11.2126-2131.1987.
6
Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids.通过高效液相色谱法分析分枝菌酸来鉴定主要的缓慢生长致病性分枝杆菌和戈登分枝杆菌。
J Clin Microbiol. 1988 Jan;26(1):50-3. doi: 10.1128/jcm.26.1.50-53.1988.
7
High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.分枝菌酸的高效液相色谱图谱作为龟分枝杆菌、偶然分枝杆菌和耻垢分枝杆菌鉴定标准
J Clin Microbiol. 1990 Sep;28(9):2094-8. doi: 10.1128/jcm.28.9.2094-2098.1990.
8
Chronic tenosynovitis of the hand due to Mycobacterium nonchromogenicum: use of high-performance liquid chromatography for identification of isolates.非产色分枝杆菌所致手部慢性腱鞘炎：利用高效液相色谱法鉴定分离株
Rev Infect Dis. 1991 Sep-Oct;13(5):857-64. doi: 10.1093/clinids/13.5.857.
9
Identification of mycobacteria by high-performance liquid chromatography.通过高效液相色谱法鉴定分枝杆菌。
J Clin Microbiol. 1991 Nov;29(11):2468-72. doi: 10.1128/jcm.29.11.2468-2472.1991.
10
Fourth report of the cooperative, open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy.分枝杆菌分类学国际工作组关于缓慢生长分枝杆菌的合作性、开放性研究的第四次报告
Int J Syst Bacteriol. 1991 Oct;41(4):463-72. doi: 10.1099/00207713-41-4-463.
Zotero 插件