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A cloned DNA probe identifies Cowdria ruminantium in Amblyomma variegatum ticks.

作者信息

Waghela S D, Rurangirwa F R, Mahan S M, Yunker C E, Crawford T B, Barbet A F, Burridge M J, McGuire T C

机构信息

Kenya Agricultural Research Institute, Kabete.

出版信息

J Clin Microbiol. 1991 Nov;29(11):2571-7. doi: 10.1128/jcm.29.11.2571-2577.1991.

DOI:10.1128/jcm.29.11.2571-2577.1991
PMID:1774264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC270375/
Abstract

Heartwater, caused by Cowdria ruminantium and transmitted by ticks of the genus Amblyomma, is a constraint to ruminant animal production in sub-Saharan Africa. This rickettsial disease could spread from endemically infected areas of sub-Saharan Africa and certain Caribbean islands to other countries, including the United States, in which Amblyomma ticks exist. To detect C. ruminantium in tick vectors and animals, we made DNA probes from C. ruminantium DNA isolated from endothelial cell cultures. Two clones were evaluated; pCS20 from Crystal Springs (Zimbabwe) strain DNA had a 1,306-bp insert, and pCR9 from Kiswani (Kenya) strain DNA had a 754-bp insert. Both DNA probes detected 1 ng of Crystal Springs DNA; however, the pCS20 probe had a 10-fold-greater ability to discriminate between C. ruminantium DNA and DNA from other organisms. Also, the pCS20 probe did not hybridize to 400 ng (highest amount tested) of DNA from bovine cells, 3 protozoa, 3 rickettsiae, and 12 bacteria. In all experiments, C. ruminantium DNA was detected in midguts from 99 of 160 Amblyomma variegatum nymphs infected as larvae and in midguts from 38 of 80 adult ticks infected as nymphs but not in midguts from control nymphs and adults. The presence of C. ruminantium in nymphs and adults was confirmed by transmission of heartwater to goats. The DNA sequences of both probes were determined; synthetic oligonucleotides from pCS20 are recommended as DNA probes for C. ruminantium.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/05a4b9899c41/jcm00047-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/06f42a0458fb/jcm00047-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/d16bb3af4e0f/jcm00047-0227-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/a162ab06be58/jcm00047-0227-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/3134b1621799/jcm00047-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/f9ec9f528a5f/jcm00047-0228-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/05a4b9899c41/jcm00047-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/06f42a0458fb/jcm00047-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/d16bb3af4e0f/jcm00047-0227-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/a162ab06be58/jcm00047-0227-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/3134b1621799/jcm00047-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/f9ec9f528a5f/jcm00047-0228-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/270375/05a4b9899c41/jcm00047-0229-a.jpg

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A cloned DNA probe identifies Cowdria ruminantium in Amblyomma variegatum ticks.
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2
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本文引用的文献

1
Experimental transmission of Cowdria ruminantium by the Gulf coast tick Amblyomma maculatum: danger of introducing heartwater and benign African theileriasis onto the American mainland.
Am J Vet Res. 1982 Jul;43(7):1279-82.
2
Observation of Rickettsia ruminantium in leukocytic cell cultures from heartwater-infected goats, sheep, and cattle.从感染心水病的山羊、绵羊和牛的白细胞培养物中观察反刍兽立克次氏体。
Am J Vet Res. 1983 Jun;44(6):1093-7.
3
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.用于可视化与固定在硝酸纤维素膜上的DNA或RNA杂交的生物素标记DNA探针的快速灵敏比色法:生物印迹法。
通过pCS20聚合酶链反应(PCR)和MAP1-B酶联免疫吸附测定(ELISA)对冈比亚羔羊和幼羊进行反刍兽埃立克体感染的纵向监测。
BMC Infect Dis. 2007 Jul 27;7:85. doi: 10.1186/1471-2334-7-85.
4
Comparative genomics of Rickettsia prowazekii Madrid E and Breinl strains.普氏立克次体马德里E株和布雷茵尔株的比较基因组学
J Bacteriol. 2004 Jan;186(2):556-65. doi: 10.1128/JB.186.2.556-565.2004.
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Novel Ehrlichia genotype detected in dogs in South Africa.在南非的狗身上检测到新型埃立克体基因型。
J Clin Microbiol. 2001 Nov;39(11):4204-7. doi: 10.1128/JCM.39.11.4204-4207.2001.
6
Antibody responses to MAP 1B and other Cowdria ruminantium antigens are down regulated in cattle challenged with tick-transmitted heartwater.在受到蜱传播的牛心水病攻击的牛中,对微管相关蛋白1B(MAP 1B)和其他反刍动物考德里氏体抗原的抗体反应受到下调。
Clin Diagn Lab Immunol. 2001 Mar;8(2):388-96. doi: 10.1128/CDLI.8.2.388-396.2001.
7
Detection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks.通过聚合酶链反应(PCR)检测钝缘蜱中牛心水病病原体反刍动物考德里氏体:该检测方法的验证及其在野外蜱虫中的应用
J Clin Microbiol. 2000 Apr;38(4):1539-44. doi: 10.1128/JCM.38.4.1539-1544.2000.
8
Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): a review.蜱虫(蜱螨亚纲:硬蜱科)病原体DNA的分子检测:综述
Exp Appl Acarol. 1999 Dec;23(12):929-60. doi: 10.1023/a:1006313803979.
9
Evidence to show that an agent that cross-reacts serologically with Cowdria ruminantium in Zimbabwe is transmitted by ticks.有证据表明,在津巴布韦与反刍兽考德里氏体发生血清交叉反应的一种病原体是由蜱传播的。
Exp Appl Acarol. 1998 Feb;22(2):111-22. doi: 10.1023/a:1006045710683.
10
Purification and characterization of the Pac1 ribonuclease of Schizosaccharomyces pombe.粟酒裂殖酵母Pac1核糖核酸酶的纯化与特性分析
Nucleic Acids Res. 1996 Jun 15;24(12):2377-86. doi: 10.1093/nar/24.12.2377.
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4045-9. doi: 10.1073/pnas.80.13.4045.
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Heartwater.
Bull Off Int Epizoot. 1968 May;70(1):329-36.
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Electron microscopy of Cowdria (Rickettsia) ruminantium (Cowdry, 1926) in the endothelial cells of the vertebrate host.
Onderstepoort J Vet Res. 1970 Mar;37(1):67-78.
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Heartwater in the Caribbean: isolation of Cowdria ruminantium from Antigua.
Vet Rec. 1985 Feb 2;116(5):121-3. doi: 10.1136/vr.116.5.121.
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Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments.使用克隆的基因组DNA片段选择性检测猪鼻支原体。
Infect Immun. 1985 Mar;47(3):827-30. doi: 10.1128/iai.47.3.827-830.1985.
8
A method for determining the Cowdria ruminantium infection rate of Amblyomma hebraeum: effects in mice injected with tick homogenates.一种测定希伯来花蜱中反刍兽考德里氏体感染率的方法:对注射蜱匀浆的小鼠的影响
Onderstepoort J Vet Res. 1985 Jun;52(2):55-61.
9
Ultrastructural features of Cowdria ruminantium in midgut epithelial cells and salivary glands of nymphal Amblyomma hebraeum.
Onderstepoort J Vet Res. 1987 Mar;54(1):87-92.
10
Demonstration of colonies of Cowdria ruminantium in midgut epithelial cells of Amblyomma variegatum.
Am J Vet Res. 1987 Mar;48(3):356-60.