使用克隆的基因组DNA片段选择性检测猪鼻支原体。
Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments.
作者信息
Taylor M A, Wise K S, McIntosh M A
出版信息
Infect Immun. 1985 Mar;47(3):827-30. doi: 10.1128/iai.47.3.827-830.1985.
Abstract
A 13-kilobase DNA fragment from a genomic library of Mycoplasma hyorhinis demonstrated specific Southern hybridization and dot hybridization when tested against a group of different mycoplasmas. This probe selectively recognized M. hyorhinis sequences in purified DNA, broth-grown organisms, and infected cell cultures, providing a direct method for differential detection of this species.
摘要
来自猪鼻支原体基因组文库的一个13千碱基的DNA片段，在与一组不同支原体进行检测时，表现出特异性的Southern杂交和点杂交。该探针能在纯化DNA、肉汤培养的菌体以及感染的细胞培养物中选择性识别猪鼻支原体序列，为该物种的鉴别检测提供了一种直接方法。
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