Du Plessis J L
Onderstepoort J Vet Res. 1985 Jun;52(2):55-61.
Amblyomma hebraeum ticks, collected in the field and individually homogenized, were injected into mice. Thirteen out of 240 ticks were shown to be infected with the heartwater agent. Antibodies against Cowdria ruminantium were detected in the sera of the mice by means of the indirect fluorescent antibody test. Giemsa-stained smears, prepared from the haemocytes of the ticks, revealed morphologically different forms of the heartwater agent. A strain of C. ruminantium, designated the Welgevonden strain, was isolated in mice from one of the infected ticks and passaged in mice for 8 generations. When inoculated intravenously, it was highly infective to mice, sheep and cattle. The murinotropism of the Welgevonden strain is compared with that of other strains previously described.
将在野外采集并单独匀浆的希伯来花蜱注射到小鼠体内。在240只蜱中，有13只被证明感染了心水病病原体。通过间接荧光抗体试验在小鼠血清中检测到了抗反刍兽考德里氏体的抗体。从蜱的血细胞制备的吉姆萨染色涂片显示了心水病病原体形态不同的形式。从一只受感染的蜱中在小鼠体内分离出一株反刍兽考德里氏体，命名为韦尔格冯登菌株，并在小鼠体内传代8代。静脉接种时，它对小鼠、绵羊和牛具有高度传染性。将韦尔格冯登菌株的嗜鼠性与先前描述的其他菌株进行了比较。
