Van Damme-Jongsten M, Rodhouse J, Gilbert R J, Notermans S
Laboratory of Water and Food Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
J Clin Microbiol. 1990 Jan;28(1):131-3. doi: 10.1128/jcm.28.1.131-133.1990.
Four synthetic oligonucleotides encoding different parts of the Clostridium perfringens enterotoxin gene were used to test the enterotoxigenicity of C. perfringens strains isolated from confirmed outbreaks of food poisoning. Of the 245 strains isolated from food and feces originating from 186 separate outbreaks, 145 (59%) gave hybridization reactions with each of the four DNA probes used, while 104 strains did not hybridize with any of the probes. There was no correlation between serotype and the presence of the enterotoxin gene, although the C. perfringens enterotoxin gene was rarely detected among nontypable strains (17%). Results show that DNA hybridization is a suitable method for the identification of C. perfringens strains which have the potential to produce enterotoxin.
使用四种编码产气荚膜梭菌肠毒素基因不同部分的合成寡核苷酸，来检测从确诊食物中毒暴发中分离出的产气荚膜梭菌菌株的产肠毒素能力。从186起单独暴发事件的食物和粪便中分离出的245株菌株中，145株（59%）与所使用的四种DNA探针中的每一种都发生了杂交反应，而104株菌株未与任何探针杂交。血清型与肠毒素基因的存在之间没有相关性，尽管在不可分型菌株中很少检测到产气荚膜梭菌肠毒素基因（17%）。结果表明，DNA杂交是鉴定具有产生肠毒素潜力的产气荚膜梭菌菌株的合适方法。
