用于检测从食物中毒暴发中分离出的产肠毒素产气荚膜梭菌菌株的合成DNA探针。
Synthetic DNA probes for detection of enterotoxigenic Clostridium perfringens strains isolated from outbreaks of food poisoning.
作者信息
Van Damme-Jongsten M, Rodhouse J, Gilbert R J, Notermans S
机构信息
Laboratory of Water and Food Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
出版信息
J Clin Microbiol. 1990 Jan;28(1):131-3. doi: 10.1128/jcm.28.1.131-133.1990.
Abstract
Four synthetic oligonucleotides encoding different parts of the Clostridium perfringens enterotoxin gene were used to test the enterotoxigenicity of C. perfringens strains isolated from confirmed outbreaks of food poisoning. Of the 245 strains isolated from food and feces originating from 186 separate outbreaks, 145 (59%) gave hybridization reactions with each of the four DNA probes used, while 104 strains did not hybridize with any of the probes. There was no correlation between serotype and the presence of the enterotoxin gene, although the C. perfringens enterotoxin gene was rarely detected among nontypable strains (17%). Results show that DNA hybridization is a suitable method for the identification of C. perfringens strains which have the potential to produce enterotoxin.
摘要
使用四种编码产气荚膜梭菌肠毒素基因不同部分的合成寡核苷酸，来检测从确诊食物中毒暴发中分离出的产气荚膜梭菌菌株的产肠毒素能力。从186起单独暴发事件的食物和粪便中分离出的245株菌株中，145株（59%）与所使用的四种DNA探针中的每一种都发生了杂交反应，而104株菌株未与任何探针杂交。血清型与肠毒素基因的存在之间没有相关性，尽管在不可分型菌株中很少检测到产气荚膜梭菌肠毒素基因（17%）。结果表明，DNA杂交是鉴定具有产生肠毒素潜力的产气荚膜梭菌菌株的合适方法。
相似文献
1
Synthetic DNA probes for detection of enterotoxigenic Clostridium perfringens strains isolated from outbreaks of food poisoning.用于检测从食物中毒暴发中分离出的产肠毒素产气荚膜梭菌菌株的合成DNA探针。
J Clin Microbiol. 1990 Jan;28(1):131-3. doi: 10.1128/jcm.28.1.131-133.1990.
2
Evaluation of synthetic DNA probes for confirmation of Clostridium perfringens enterotoxin gene PCR products.用于确认产气荚膜梭菌肠毒素基因PCR产物的合成DNA探针的评估
Southeast Asian J Trop Med Public Health. 2005 May;36(3):663-72.
3
Detection of Clostridium perfringens enterotoxin gene by the polymerase chain reaction amplification procedure.采用聚合酶链反应扩增法检测产气荚膜梭菌肠毒素基因。
Int J Food Microbiol. 1992 Sep;17(1):47-55. doi: 10.1016/0168-1605(92)90018-x.
4
PCR identification of the plasmid-borne enterotoxin gene (cpe) in Clostridium perfringens strains isolated from food poisoning outbreaks.从食物中毒暴发中分离出的产气荚膜梭菌菌株中质粒携带的肠毒素基因（cpe）的聚合酶链反应鉴定
Int J Med Microbiol. 2004 Oct;294(4):261-5. doi: 10.1016/j.ijmm.2004.03.004.
5
Genetic diversity of Clostridium perfringens type A isolates from animals, food poisoning outbreaks and sludge.来自动物、食物中毒暴发事件及污泥的A型产气荚膜梭菌分离株的遗传多样性
BMC Microbiol. 2006 May 31;6:47. doi: 10.1186/1471-2180-6-47.
6
Identification and Characterization of a New Enterotoxin Produced by Clostridium perfringens Isolated from Food Poisoning Outbreaks.从食物中毒暴发中分离出的产气荚膜梭菌产生的一种新型肠毒素的鉴定与特性分析
PLoS One. 2015 Nov 19;10(11):e0138183. doi: 10.1371/journal.pone.0138183. eCollection 2015.
7
Clostridium perfringens and foodborne infections.产气荚膜梭菌与食源性感染。
Int J Food Microbiol. 2002 Apr 5;74(3):195-202. doi: 10.1016/s0168-1605(01)00680-8.
8
The identification and characterization of Clostridium perfringens by real-time PCR, location of enterotoxin gene, and heat resistance.通过实时聚合酶链反应对产气荚膜梭菌进行鉴定和特征分析、肠毒素基因定位及耐热性研究
Foodborne Pathog Dis. 2008 Oct;5(5):629-39. doi: 10.1089/fpd.2007.0066.
9
10
Clostridium perfringens type A strains carrying a plasmid-borne enterotoxin gene (genotype IS1151-cpe or IS1470-like-cpe) as a common cause of food poisoning.携带质粒介导的肠毒素基因（基因型IS1151-cpe或IS1470样-cpe）的A型产气荚膜梭菌菌株是食物中毒的常见原因。
J Clin Microbiol. 2008 Jan;46(1):371-3. doi: 10.1128/JCM.01650-07. Epub 2007 Nov 14.
引用本文的文献
1
Genotyping of Isolates from Domestic Livestock in Saudi Arabia.沙特阿拉伯家畜分离株的基因分型。
Biomed Res Int. 2020 Mar 23;2020:9035341. doi: 10.1155/2020/9035341. eCollection 2020.
2
Antibiotic Sensitivity of Clostridium perfringens Isolated From Faeces in Tabriz, Iran.从伊朗大不里士粪便中分离出的产气荚膜梭菌的抗生素敏感性
Jundishapur J Microbiol. 2015 Jul 25;8(7):e20863. doi: 10.5812/jjm.20863v2. eCollection 2015 Jul.
3
Enterotoxigenic Clostridium perfringens: detection and identification.产肠毒素梭状芽孢杆菌：检测与鉴定。
Microbes Environ. 2012;27(4):343-9. doi: 10.1264/jsme2.me12002. Epub 2012 Apr 14.
4
Genetic diversity of Clostridium perfringens type A isolates from animals, food poisoning outbreaks and sludge.来自动物、食物中毒暴发事件及污泥的A型产气荚膜梭菌分离株的遗传多样性
BMC Microbiol. 2006 May 31;6:47. doi: 10.1186/1471-2180-6-47.
5
Enumeration and isolation of cpe-positive Clostridium perfringens spores from feces.从粪便中计数并分离出细胞病变效应阳性的产气荚膜梭菌孢子。
J Clin Microbiol. 2004 Sep;42(9):3992-7. doi: 10.1128/JCM.42.9.3992-3997.2004.
6
Methodologies for the characterization of microbes in industrial environments: a review.工业环境中微生物表征的方法：综述
J Ind Microbiol Biotechnol. 2003 Jun;30(6):327-56. doi: 10.1007/s10295-003-0056-y. Epub 2003 May 23.
7
Enterotoxigenicity and genetic relatedness of Clostridium perfringens isolates from retail foods in the United States.美国零售食品中产气荚膜梭菌分离株的产肠毒素能力及遗传相关性
Appl Environ Microbiol. 2003 Mar;69(3):1642-6. doi: 10.1128/AEM.69.3.1642-1646.2003.
8
Molecular epidemiology of Clostridium perfringens related to food-borne outbreaks of disease in Finland from 1984 to 1999.1984年至1999年芬兰与食源性疾病暴发相关的产气荚膜梭菌分子流行病学
Appl Environ Microbiol. 2002 Aug;68(8):3744-9. doi: 10.1128/AEM.68.8.3744-3749.2002.
9
Evidence for antibiotic induced Clostridium perfringens diarrhoea.抗生素诱发产气荚膜梭菌腹泻的证据。
J Clin Pathol. 2001 Oct;54(10):748-51. doi: 10.1136/jcp.54.10.748.
10
Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test.采用双重聚合酶链反应和玻片乳胶凝集试验检测食品和粪便样本中的产肠毒素性产气荚膜梭菌。
Appl Environ Microbiol. 1997 Nov;63(11):4232-6. doi: 10.1128/aem.63.11.4232-4236.1997.
本文引用的文献
1
OBSERVATIONS ON THE FLORA OF THE ALIMENTARY TRACT OF ANIMALS AND FACTORS AFFECTING ITS COMPOSITION.关于动物消化道菌群及其组成影响因素的观察
J Pathol Bacteriol. 1965 Jan;89:95-122.
2
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
3
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
4
Improved culture techniques and sporulation medium for enterotoxin production by Clostridium perfringens type A.用于A型产气荚膜梭菌产生肠毒素的改良培养技术和芽孢形成培养基。
Taiwan Yi Xue Hui Za Zhi. 1974 Jul;73(7):404-9.
5
Homology between enterotoxin protein and spore structural protein in Clostridium perfringens type A.A型产气荚膜梭菌中肠毒素蛋白与芽孢结构蛋白之间的同源性。
Eur J Biochem. 1973 Nov 15;39(2):393-401. doi: 10.1111/j.1432-1033.1973.tb03137.x.
6
Sporulation and enterotoxin production by mutants of Clostridium perfringens.产气荚膜梭菌突变体的芽孢形成与肠毒素产生
J Bacteriol. 1972 Apr;110(1):378-91. doi: 10.1128/jb.110.1.378-391.1972.
7
Improved medium for sporulation of Clostridium perfringens.用于产气荚膜梭菌芽孢形成的改良培养基。
Appl Microbiol. 1968 Jan;16(1):82-9. doi: 10.1128/am.16.1.82-89.1968.
8
The amino acid sequence of the enterotoxin from Clostridium perfringens type A.A型产气荚膜梭菌肠毒素的氨基酸序列
FEBS Lett. 1985 Mar 25;182(2):479-84. doi: 10.1016/0014-5793(85)80358-6.
9
Enterotoxin synthesis by nonsporulating cultures of Clostridium perfringens.产气荚膜梭菌非芽孢形成培养物的肠毒素合成
Appl Environ Microbiol. 1986 Sep;52(3):407-12. doi: 10.1128/aem.52.3.407-412.1986.
10
Development and application of an enzyme linked immunosorbent assay for Clostridium perfringens type A enterotoxin.A型产气荚膜梭菌肠毒素酶联免疫吸附测定法的开发与应用
J Clin Pathol. 1985 Feb;38(2):222-8. doi: 10.1136/jcp.38.2.222.
Zotero 插件