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A comparison of the direct immunobead test and other tests for sperm antibodies detection.

作者信息

Dondero F, Lenzi A, Gandini L, Lombardo F, Culasso F

机构信息

Laboratorio di Immunologia della Riproduzione e Seminologia, V Clinica Medica, Università di Roma, La Sapienza, Italy.

出版信息

J Endocrinol Invest. 1991 Jun;14(6):443-9. doi: 10.1007/BF03346835.

DOI:10.1007/BF03346835
PMID:1774440
Abstract

The authors report the results of a correlation study between the direct Immunobead Test (d-IBT) and other techniques for antisperm antibodies detection. The Gelatin Agglutination Test (GAT) and Tray Agglutination Test (TAT) were used to detect antibodies in blood serum and seminal plasma ("indirect methods"). The Direct IgG Mixed Antiglobulin Reaction Test (d-MAR test) was used to detect sperm antibodies bound to the sperm surface ("direct method"). A good concordance between the methods, measured by phi and K tests, was found and satisfactory mathematical models were established by regression analyses.

摘要

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本文引用的文献

1
Methods for the detection of antibodies against mammalian spermatozoa. II. A gelatin agglutination test.检测抗哺乳动物精子抗体的方法。II. 明胶凝集试验。
Fertil Steril. 1952 Sep-Oct;3(5):430-8. doi: 10.1016/s0015-0282(16)31026-3.
2
Ability of antibody-bound human sperm to penetrate zona-free hamster ova in vitro.体外抗体结合的人精子穿透无透明带仓鼠卵的能力。
Fertil Steril. 1981 Dec;36(6):778-83. doi: 10.1016/s0015-0282(16)45925-x.
3
Correlation between regional specificity of antisperm antibodies to the spermatozoan surface and complement-mediated sperm immobilization.
J Endocrinol Invest. 1995 Jun;18(6):468-83. doi: 10.1007/BF03349748.
抗精子抗体对精子表面的区域特异性与补体介导的精子制动之间的相关性。
Am J Reprod Immunol (1980). 1982 Aug;2(4):222-4. doi: 10.1111/j.1600-0897.1982.tb00170.x.
4
A simple and sensitive micro-method for demonstration of sperm-agglutinating activity in serum from infertile men and women.一种用于检测不孕男性和女性血清中精子凝集活性的简单且灵敏的微量方法。
Acta Obstet Gynecol Scand Suppl. 1974;36:21-9. doi: 10.3109/00016347409156399.
5
Correlation between the direct IgG MAR test (mixed antiglobulin reaction test) and seminal analysis in men from infertile couples.不育夫妇男性中直接IgG MAR试验（混合抗球蛋白反应试验）与精液分析之间的相关性。
Fertil Steril. 1985 Sep;44(3):390-5. doi: 10.1016/s0015-0282(16)48865-5.
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Anti-sperm antibodies, detected by agglutination, immobilization, microcytotoxicity and immunobead-binding assays.
J Reprod Immunol. 1985 Dec;8(4):279-99. doi: 10.1016/0165-0378(85)90003-8.
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Clarifying antibody-mediated infertility.阐明抗体介导的不孕症。
Am J Reprod Immunol Microbiol. 1985 Apr;7(4):148. doi: 10.1111/j.1600-0897.1985.tb00343.x.
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The immunoglobulin class of antispermatozoal antibodies in serum.
Am J Reprod Immunol Microbiol. 1985 Apr;7(4):143-7. doi: 10.1111/j.1600-0897.1985.tb00342.x.
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Detection of sperm antibodies in semen using the immunobead test: a survey of 813 consecutive patients.
Am J Reprod Immunol Microbiol. 1985 Mar;7(3):118-23. doi: 10.1111/j.1600-0897.1985.tb00269.x.
10
Antibodies to spermatozoa: XI. The use of immunobeads for the detection of sperm antibodies in serum.
Am J Reprod Immunol Microbiol. 1985 Oct;9(2):62-6. doi: 10.1111/j.1600-0897.1985.tb00277.x.