Bronson R A, Cooper G W, Rosenfeld D L
Am J Reprod Immunol (1980). 1982 Aug;2(4):222-4. doi: 10.1111/j.1600-0897.1982.tb00170.x.
Sera from men at risk for immunity to spermatozoa were screened for antisperm antibodies by immunobead binding following passive antibody transfer to antibody-free sperm of fertile donors. The percent motile sperm after incubation in diluted antibody positive serum in the presence of complement was compared with the regional distribution of immunoglobulins bound to the sperm surface. The extent of complement-mediated sperm immobilization varied with immunoglobulin class and with the location of antibody bound to the sperm surface. Tests utilizing complement-mediated immobilization of sperm are insensitive to the presence of antibodies of IgG and IgA classes that are directed against the head, the distal one-fifth of the sperm tail principal piece, or the tail end piece. A high degree of immobilization was found only when IgG binding occurred on the distal two-fifths to three-fifths of the principal piece of the tail or when IgM bound to the sperm tail end piece. (Am J Reprod Immunol. 2:222-224.)
在将被动抗体转移至可育供体的无抗体精子后，通过免疫珠结合法对有精子免疫风险男性的血清进行抗精子抗体筛查。将在补体存在的情况下，在稀释的抗体阳性血清中孵育后的活动精子百分比与结合在精子表面的免疫球蛋白区域分布进行比较。补体介导的精子固定程度随免疫球蛋白类别以及抗体在精子表面的结合位置而变化。利用补体介导的精子固定进行的检测，对针对精子头部、精子尾部主段远端五分之一或尾端的IgG和IgA类抗体不敏感。仅当IgG结合发生在尾部主段远端五分之二至五分之三时，或当IgM结合到精子尾端时，才会发现高度固定。（《美国生殖免疫学杂志》。2:222 - 224。）
