Andreou E, Mahmoud A, Vermeulen L, Schoonjans F, Comhaire F
University Hospital Ghent, Department of Internal Medicine, Belgium.
Hum Reprod. 1995 Jan;10(1):125-31. doi: 10.1093/humrep/10.1.125.
Since there is no gold standard for the diagnosis of immunological infertility, comparison between different methods and of their results with biological tests for the detection of antisperm antibodies must be used in defining the most reliable and clinically relevant method. We have evaluated the results of direct (n = 100) and indirect tests in serum (n = 140) and seminal plasma (n = 100) using the SpermMAR and Immunobead (IB) techniques for the detection of sperm antibodies of the immunoglobulin (Ig) classes G, A and M, and we have compared the results with those of the tray agglutination test and the adenosine triphosphate release cytotoxicity test. Results indicate the higher specificity and sensitivity of the SpermMAR results for IgG and IgA, as compared to the outcome of the IB test. It appears that the IB test for IgA may detect non-specific antibodies in serum. Little information is obtained from the tray agglutination test in seminal plasma, and the indirect SpermMar test for IgG in seminal plasma was the only independent variable associated with the outcome of the former test. Since the SpermMAR tests for IgG and IgA are more accurate and biologically relevant, as well as easier to perform than the respective IB tests, the former must be considered the method of first choice.
由于免疫性不孕的诊断尚无金标准，在确定最可靠且临床相关的方法时，必须对不同方法及其结果与检测抗精子抗体的生物学检测进行比较。我们使用精子膜抗体免疫珠试验（SpermMAR）和免疫珠（IB）技术评估了直接检测（n = 100）以及血清（n = 140）和精浆（n = 100）间接检测中免疫球蛋白（Ig）G、A和M类精子抗体的结果，并将结果与平板凝集试验和三磷酸腺苷释放细胞毒性试验的结果进行了比较。结果表明，与IB试验结果相比，SpermMAR试验对IgG和IgA具有更高的特异性和敏感性。似乎用于检测IgA的IB试验可能会检测出血清中的非特异性抗体。从精浆中的平板凝集试验获得的信息很少，精浆中IgG的间接SpermMar试验是与前一项试验结果相关的唯一独立变量。由于针对IgG和IgA的SpermMAR试验比各自的IB试验更准确、更具生物学相关性且更易于操作，因此前者应被视为首选方法。
