Science. 1984 Mar 23;223(4642):1265-8. doi: 10.1126/science.223.4642.1265.
An active maize Adhl-F gene, a Ds-induced mutant of this gene, and two independent Ac-induced revertant alleles have been isolated. The Ds mutant differs from the progenitor allele in having a 405-base pair insertion flanked by a direct repeat of 8 bp. The repeat is a duplication of the 8 bp existing at the point of insertion in the 5' untranslated region of the gene. The insertion sequence is AT-rich (A, adenine; T, thymine) and has 11-bp inverted repeat sequences at its termini. In the revertants the insertion with its inverted repeats is deleted, but the 8-bp direct repeats remain in modified form. These results establish that the 405-bp sequence is a Ds element. The Adh1 messenger RNA level is low in the Ds mutant, and it appears that new sites for transcription initiation or RNA processing or both are used. There are at least 30 sequences in the maize genome related to the Ds element.
已分离到一个活跃的玉米 Adhl-F 基因、该基因的一个 Ds 诱导突变体以及两个独立的 Ac 诱导回复突变等位基因。Ds 突变体与前体等位基因的不同之处在于其 405 个碱基对的插入序列由 8 个碱基对的直接重复序列侧翼包围。重复序列是基因 5'非翻译区插入点处存在的 8 个碱基对的重复。插入序列富含 AT（A，腺嘌呤；T，胸腺嘧啶），并且在其末端具有 11 个碱基对的反向重复序列。在回复突变体中，插入序列及其反向重复序列被删除，但 8 个碱基对的直接重复序列仍以修饰形式存在。这些结果表明，405 个碱基对序列是一个 Ds 元件。Ds 突变体中的 Adh1 信使 RNA 水平较低，似乎使用了新的转录起始或 RNA 加工或两者的位点。在玉米基因组中，至少有 30 个序列与 Ds 元件相关。
