Bennetzen J L, Swanson J, Taylor W C, Freeling M
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4125-8. doi: 10.1073/pnas.81.13.4125.
A genetically unstable mutant allele of maize alcohol dehydrogenase-1 (Adh1) causes reduced levels of messenger RNA. This mutant was derived from a Robertson's mutator genetic background and previously was shown to be associated with an approximately 1.5-kilobase insertion. This report compares a genomic clone of the allele, Adh1-S3034, with a clone of its progenitor nonmutant allele, Adh1-S. No rearrangements of either recombinant molecule occurred during the cloning process. The mutant differs from its progenitor allele by the insertion of 1.4 kilobases of new DNA. Sequence determination of the insertion borders localized it to intron-1, 73 base pairs downstream from the 5' exon/intron junction in the Adh1-S gene. Insertion of the 1.4-kilobase element was associated with a 9-base-pair direct duplication of intron sequence. We suspect that this insertion, Mu1, affects message levels by depressing RNA processing or transcription.
玉米乙醇脱氢酶-1（Adh1）的一个遗传不稳定突变等位基因导致信使核糖核酸水平降低。该突变体源自罗伯逊突变体遗传背景，此前已证明与一个约1.5千碱基的插入有关。本报告将该等位基因Adh1-S3034的基因组克隆与其祖先非突变等位基因Adh1-S的克隆进行了比较。在克隆过程中，两个重组分子均未发生重排。该突变体与其祖先等位基因的区别在于插入了1.4千碱基的新DNA。插入边界的序列测定将其定位到内含子-1，位于Adh1-S基因5'外显子/内含子交界处下游73个碱基对处。1.4千碱基元件的插入与内含子序列的9个碱基对直接重复相关。我们怀疑这种插入，即Mu1，通过抑制RNA加工或转录来影响信息水平。
