Eid Manal, El-Brashy Amina, Aly Fatma, Talaat Wael
Mansoura University, Faculty of Pharmacy, Department of Analytical Chemistry, Mansoura 35516, Egypt.
J AOAC Int. 2007 Jul-Aug;90(4):941-7.
A simple and sensitive fluorometric method for determination of ketorolac tromethamine was studied. The method depends on oxidation of the drug with cerium(IV) and subsequent monitoring of the fluorescence of the induced cerium(III) at lambda(em) 365 nm after excitation at 255 nm. Different variables affecting the reaction conditions, such as the concentrations of cerium(IV), sulfuric acid concentration, reaction time, and temperature, were carefully studied and optimized. Under the optimum conditions, a linear relationship was found between the relative fluorescence intensity and the concentration of the investigated drug in the range of 0.1-0.8 microg/mL. No interferences could be observed from the excipients commonly present in dosage forms. The proposed method was successfully applied to the analysis of the investigated drug in its pure form, pharmaceutical preparations, and biological fluids with good accuracy and precision. The recoveries for pharmaceutical formulations ranged from 99.8-101.0 +/- 0.6% for tablets, 98.5-101.0 +/- 1.0% for ampoules, and 99.0-100.5 +/- 0.7% for eye drops. The results obtained by the proposed method were satisfactory compared with those obtained by the official method. The recoveries for biological fluids were 99.1-100.4 +/- 0.7 and 99.0-100.0 +/- 0.5% for plasma and urine, respectively.
研究了一种测定酮咯酸氨丁三醇的简单灵敏的荧光分析法。该方法基于药物与铈（IV）的氧化反应，随后在255nm激发后监测在365nm处诱导产生的铈（III）的荧光。仔细研究并优化了影响反应条件的不同变量，如铈（IV）的浓度、硫酸浓度、反应时间和温度。在最佳条件下，在所研究药物浓度为0.1 - 0.8μg/mL范围内，相对荧光强度与药物浓度之间呈线性关系。未观察到剂型中常见辅料的干扰。所提出的方法成功应用于所研究药物的纯品、药物制剂和生物流体的分析，具有良好的准确度和精密度。药物制剂的回收率：片剂为99.8 - 101.0±0.6%，安瓿剂为98.5 - 101.0±1.0%，眼药水为99.0 - 100.5±0.7%。与官方方法相比，所提出方法得到的结果令人满意。生物流体的回收率：血浆为99.1 - 100.4±0.7%，尿液为99.0 - 100.0±0.5%。
