Razak O A
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Alexandria, Egypt.
J Pharm Biomed Anal. 1998 Nov;18(3):359-65. doi: 10.1016/s0731-7085(98)00093-4.
Two simple sensitive and accurate methods have been developed for the determination of ritodrine hydrochloride in bulk and pharmaceutical preparations. The first method involves the direct measurement of the native fluorescence of the drug in the concentration range 4-9 microg ml(-1), the second method is based on the oxidation of ritodrine HCl with cerium(IV) followed either by spectrophotometric or fluorimetric measurement in the concentration ranges 0.5-1.0 and 0.05-0.1 microg ml(-1), respectively. The interference of various formulation excipients was examined. The reliability of the proposed methods was checked at three different concentrations; the standard deviation varied from 2.7 x 10(-3)-0.109. The described methods have been applied to the determination of ritodrine HCl in tablets and ampoules. The assay results showed insignificant difference with those of the official USP 23 HPLC method.
已开发出两种简单、灵敏且准确的方法用于测定原料药及药物制剂中的盐酸利托君。第一种方法是直接测量浓度范围为4 - 9微克/毫升的药物天然荧光，第二种方法基于盐酸利托君与铈（IV）的氧化反应，随后分别在浓度范围为0.5 - 1.0微克/毫升和0.05 - 0.1微克/毫升下进行分光光度法或荧光法测量。考察了各种制剂辅料的干扰情况。在三种不同浓度下检验了所提出方法的可靠性；标准偏差在2.7×10⁻³ - 0.109之间变化。所描述的方法已应用于片剂和安瓿剂中盐酸利托君的测定。测定结果与美国药典23版官方高效液相色谱法的结果无显著差异。
