Darwish Ibrahim A, Khedr Alaa S, Askal Hassan F, Mahmoud Ramadan M
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
Farmaco. 2005 Jun-Jul;60(6-7):555-62. doi: 10.1016/j.farmac.2005.04.003.
A simple and sensitive fluorimetric method for determination of antiviral drugs: ribavirin, acyclovir, and amantadine hydrochloride has been developed. The method was based on the oxidation of these drugs by cerium(IV) in presence of perchloric acid and subsequent monitoring the fluorescence of the induced cerium(III) at lambdaexcitation 255 and lambdaemission 355 nm. Different variables affecting the reaction conditions such as the concentrations of cerium(IV), type and concentration of acid medium, reaction time, temperature, and the diluting solvents were carefully studied and optimized. Under the optimum conditions, linear relationships with good correlation coefficients (0.9978-0.9996) were found between the relative fluorescence intensity and the concentrations of the investigated drugs in the range of 50-1400 ng ml-1. The assay limits of detection and quantitation were 20-49, and 62-160 ng ml-1, respectively. The precision of the method was satisfactory; the values of relative standard deviations did not exceed 1.58%. No interference could be observed from the excipients commonly present in dosage forms. The proposed method was successfully applied to the analysis of the investigated drugs in pure and pharmaceutical dosage forms with good accuracy and precision; the recovery percentages ranged from 99.2 to 101.2+/-0.48-1.30%. The results obtained by the proposed fluorimetric method were comparable with those obtained by the official method stated in the United States Pharmacopoeia.
已开发出一种用于测定抗病毒药物（利巴韦林、阿昔洛韦和盐酸金刚烷胺）的简单且灵敏的荧光分析法。该方法基于在高氯酸存在下铈（IV）氧化这些药物，随后监测在激发波长255nm和发射波长355nm处诱导产生的铈（III）的荧光。仔细研究并优化了影响反应条件的不同变量，如铈（IV）的浓度、酸性介质的类型和浓度、反应时间、温度以及稀释溶剂。在最佳条件下，在所研究药物浓度为50 - 1400 ng ml-1范围内，相对荧光强度与药物浓度之间呈现出具有良好相关系数（0.9978 - 0.9996）的线性关系。检测限和定量限分别为20 - 49 ng ml-1和62 - 160 ng ml-1。该方法的精密度令人满意；相对标准偏差值不超过1.58%。未观察到剂型中常见辅料的干扰。所提出的方法成功应用于纯品和药物剂型中所研究药物的分析，具有良好的准确度和精密度；回收率范围为99.2%至101.2%±0.48% - 1.30%。所提出的荧光分析法获得的结果与美国药典中规定的法定方法获得的结果相当。
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