Ko Chang-Beom, Woo Young-Min, Lee Dong Ju, Lee Myung-Chul, Kim Cheol Soo
Department of Plant Biotechnology and Agricultural Plant Stress Research Center, Chonnam National University, 300 Yongbong-dong, Buk-gu, Kwangju 500-757, South Korea.
Plant Physiol Biochem. 2007 Sep;45(9):722-8. doi: 10.1016/j.plaphy.2007.07.010. Epub 2007 Jul 25.
We conducted a genetic yeast screen to identify Thermo-tolerance genes (TTOs) in maize kernel cDNA library. During the screening, we identified a maize clone (TTO6) that seemed to confer elevated heat tolerance in comparison to control cells. TTO6 cDNA (GenBank accession no. AY103785) encodes an 11-kDa protein which is 69% similarity to the Arabidopsis GASA4 gene. To further examine heat tolerance in Arabidopsis, we functionally characterized the GASA4 gene and found that heat induced GASA4 expression. Constitutive expression of GASA4 in Arabidopsis led to elevated heat tolerance in transgenic lines. Interestingly, endoplasmic reticulum chaperone expression analysis suggests that GASA4 influences BiP gene expression during heat stress.
我们进行了一项遗传酵母筛选，以在玉米籽粒cDNA文库中鉴定耐热基因（TTOs）。在筛选过程中，我们鉴定出一个玉米克隆（TTO6），与对照细胞相比，它似乎能赋予更高的耐热性。TTO6 cDNA（GenBank登录号AY103785）编码一种11 kDa的蛋白质，与拟南芥GASA4基因有69%的相似性。为了进一步研究拟南芥的耐热性，我们对GASA4基因进行了功能表征，发现热诱导GASA4表达。在拟南芥中组成型表达GASA4导致转基因株系的耐热性提高。有趣的是，内质网伴侣表达分析表明，GASA4在热胁迫期间影响BiP基因的表达。
