Bouteraa Mohamed Taieb, Ben Romdhane Walid, Wiszniewska Alina, Baazaoui Narjes, Ben Hsouna Anis, Kačániová Miroslava, Hassairi Afif, Ben Saad Rania
Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, University of Sfax, B.P "1177", 3018 Sfax, Tunisia.
Faculty of Sciences of Bizerte UR13ES47, University of Carthage, BP W, 7021 Bizerte, Tunisia.
Physiol Mol Biol Plants. 2025 May;31(5):739-751. doi: 10.1007/s12298-025-01608-z. Epub 2025 Jun 10.
The gene family () comprises plant-specific genes that play crucial roles in plant development and defense mechanisms against a plethora of abiotic and biotic stressors. As revealed in our previous study, transcripts from durum wheat are induced by a range of stressors and its heterologous expression improved stress tolerance in yeast cells. Here we have focused on role in its host species, var. durum. We examined the expression profile of the gene and found out that it is upregulated in response to environmental stimuli and phytohormones, such as SA and IAA, indicating that the gene may control stress and hormone signaling pathways in durum wheat. We subsequently engineered marker-free transgenic durum wheat lines overexpressing the gene, which exhibited improved tolerance to drought and salt stress and yielded more than non-transgenic plants (NT). regulated both scavenging capacity of the antioxidant enzyme system and the activation of five stress-related genes that act as positive regulators of salt or drought stress tolerance. In light of our results, emerges as a promising novel gene with potential for further functional analysis and exploitation in molecular breeding to enhance environmental stress tolerance in grain crops.
The online version contains supplementary material available at 10.1007/s12298-025-01608-z.
基因家族（）由植物特有的基因组成，这些基因在植物发育以及抵御多种非生物和生物胁迫的防御机制中发挥着关键作用。正如我们之前的研究所揭示的，硬粒小麦的 转录本受到一系列胁迫因子的诱导，其异源表达提高了酵母细胞的胁迫耐受性。在这里，我们重点研究了 基因在其宿主物种硬粒小麦中的作用。我们检测了 基因的表达谱，发现它在响应环境刺激和植物激素（如SA和IAA）时上调，这表明 基因可能控制硬粒小麦中的胁迫和激素信号通路。随后，我们构建了过表达 基因的无标记转基因硬粒小麦株系，这些株系表现出对干旱和盐胁迫的耐受性提高，并且产量高于非转基因植株（NT）。 调节了抗氧化酶系统的清除能力以及五个作为盐或干旱胁迫耐受性正调控因子的胁迫相关基因的激活。根据我们的研究结果， 基因成为一个有前景的新基因，具有进一步进行功能分析和在分子育种中加以利用以提高谷类作物环境胁迫耐受性的潜力。
在线版本包含可在10.1007/s12298-025-01608-z获取的补充材料。
