用于从撒哈拉以南非洲临床分离株中鉴定肺炎球菌荚膜血清型的序列多重聚合酶链反应
Sequential multiplex PCR for identifying pneumococcal capsular serotypes from South-Saharan African clinical isolates.
作者信息
Morais Luis, Carvalho Maria da Glória, Roca Anna, Flannery Brendan, Mandomando Inacio, Soriano-Gabarró Montserrat, Sigauque Betuel, Alonso Pedro, Beall Bernard
机构信息
Centro de Investigação em Saúde da Manhiça (CISM), Ministerio de Saúde, CP1929 Maputo, Mozambique.
Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
出版信息
J Med Microbiol. 2007 Sep;56(Pt 9):1181-1184. doi: 10.1099/jmm.0.47346-0.
A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8%) of 153 isolates; 126 (82.4%) were identified within two reactions. This approach in developing countries would require minimal training and could provide useful serotype information without requiring transport of specimens.
一种用于肺炎球菌血清分型的连续多重PCR方法被重新设计，用于检测莫桑比克儿童的153株临床分离株。该方法在153株分离株中的139株（90.8%）中鉴定出了血清型；126株（82.4%）在两个反应内被鉴定出来。这种方法在发展中国家所需的培训极少，并且无需运送标本就能提供有用的血清型信息。
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