用于确定从巴西儿童分离出的肺炎球菌荚膜血清型的序列多重聚合酶链反应

Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children.

作者信息

Dias Cícero A, Teixeira Lúcia Martins, Carvalho Maria da Glória, Beall Bernard

机构信息

Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.

Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, and Hospital Mãe de Deus, Porto Alegre, Rio Grande do Sul, Brazil.

出版信息

J Med Microbiol. 2007 Sep;56(Pt 9):1185-1188. doi: 10.1099/jmm.0.47347-0.

DOI:10.1099/jmm.0.47347-0

PMID:17761481

Abstract

Capsular serotype surveillance of clinical isolates of Streptococcus pneumoniae is essential for evaluation of the potential impact of introducing multivalent capsular serotype-based vaccines in Latin America. Here, a previously described sequential multiplex PCR method was revised for optimal targeting of prevalent serotypes in Latin America. The revised protocol successfully serotyped 139/147 pneumococci (94.6%) from Brazilian children, demonstrating a labour-efficient, accurate method requiring only conventional PCR capability.

摘要

对肺炎链球菌临床分离株进行荚膜血清型监测，对于评估在拉丁美洲引入基于多价荚膜血清型的疫苗的潜在影响至关重要。在此，对先前描述的序列多重PCR方法进行了修订，以最佳靶向拉丁美洲流行的血清型。修订后的方案成功地对来自巴西儿童的139/147株肺炎球菌（94.6%）进行了血清分型，证明了一种仅需常规PCR能力的高效、准确的方法。