Kou Xing-Ming, Xu Min, Gu Yong-Zuo
Faculty of Chemistry, Sichuan University, Chengdu 610064, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Jun;27(6):1197-200.
An inductively coupled plasma mass spectrometry (ICP-MS) for determination of the contents of 8 trace heavy metal elements in cortex Phellodendron chinense after microwave-assisted digestion of the sample has been developed. The accuracy of the method was evaluated by the analysis of corresponding trace heavy metal elements in standard reference materials (GBW 07604 and GBW 07605). By applying the proposed method, the contents of 8 trace heavy metal elements in cortex Phellodendron chinense cultivated in different areas (in Bazhong, Yibin and Yingjing, respectively) of Sichuan and different growth period (6, 8 and 10 years of samples from Yingjing) were determined. The relative standard deviation (RSD) is in the range of 3.2%-17.8% and the recoveries of standard addition are in the range of 70%-120%. The results of the study indicate that the proposed method has the advantages of simplicity, speediness and sensitivity. It is suitable for the determination of the contents of 8 trace heavy metal elements in cortex Phellodendron chinense. The results also show that the concentrations of 4 harmful trace heavy metal elements As, Cd, Hg and Pb in cortex Phellodendron chinense are all lower than the limits of Chinese Pharmacopoeia and Green Trade Standard for Importing and Exporting Medicinal Plant and Preparation. Therefore, the cortex Phellodendron chinense is fit for use as medicine and export.
建立了一种电感耦合等离子体质谱法（ICP-MS），用于测定黄柏样品经微波消解后8种痕量重金属元素的含量。通过分析标准参考物质（GBW 07604和GBW 07605）中相应的痕量重金属元素来评估该方法的准确性。应用该方法测定了四川不同地区（分别为巴中、宜宾和荥经）种植的黄柏以及不同生长时期（荥经6年、8年和10年生样品）中8种痕量重金属元素的含量。相对标准偏差（RSD）在3.2% - 17.8%范围内，加标回收率在70% - 120%范围内。研究结果表明，该方法具有简便、快速、灵敏的优点，适用于黄柏中8种痕量重金属元素含量的测定。结果还表明，黄柏中4种有害痕量重金属元素砷、镉、汞和铅的含量均低于《中国药典》以及《药用植物及制剂进出口绿色行业标准》的限值。因此，黄柏适合入药和出口。
