Shin Dong Hae, Hou Jingtong, Chandonia John-Marc, Das Debanu, Choi In-Geol, Kim Rosalind, Kim Sung-Hou
College of Pharmacy, Ewha Womans University, Seoul, Korea.
J Struct Funct Genomics. 2007 Sep;8(2-3):99-105. doi: 10.1007/s10969-007-9025-4. Epub 2007 Sep 2.
Advances in sequence genomics have resulted in an accumulation of a huge number of protein sequences derived from genome sequences. However, the functions of a large portion of them cannot be inferred based on the current methods of sequence homology detection to proteins of known functions. Three-dimensional structure can have an important impact in providing inference of molecular function (physical and chemical function) of a protein of unknown function. Structural genomics centers worldwide have been determining many 3-D structures of the proteins of unknown functions, and possible molecular functions of them have been inferred based on their structures. Combined with bioinformatics and enzymatic assay tools, the successful acceleration of the process of protein structure determination through high throughput pipelines enables the rapid functional annotation of a large fraction of hypothetical proteins. We present a brief summary of the process we used at the Berkeley Structural Genomics Center to infer molecular functions of proteins of unknown function.
序列基因组学的进展导致了大量源自基因组序列的蛋白质序列的积累。然而，基于目前用于检测与已知功能蛋白质序列同源性的方法，无法推断其中很大一部分蛋白质的功能。三维结构对于推断未知功能蛋白质的分子功能（物理和化学功能）可能具有重要影响。全球的结构基因组学中心一直在确定许多未知功能蛋白质的三维结构，并根据其结构推断出它们可能的分子功能。通过高通量流程成功加速蛋白质结构测定过程，并结合生物信息学和酶活性测定工具，能够对很大一部分假设蛋白质进行快速功能注释。我们简要总结了伯克利结构基因组学中心用于推断未知功能蛋白质分子功能的过程。
