Fu Elain, Ramsey Stephen A, Yager Paul
Department of Bioengineering, University of Washington, Seattle, WA 98195, United States.
Anal Chim Acta. 2007 Sep 5;599(1):118-23. doi: 10.1016/j.aca.2007.07.056. Epub 2007 Jul 31.
We demonstrate the resonance wavelength-dependent signal of colloidal gold nanoparticles adsorbed to a planar gold surface in surface plasmon resonance (SPR)-based detection. Experimental measurements of the SPR signal as a function of particle surface coverage are presented for three different resonance wavelengths. The SPR signal due to the colloidal gold nanoparticles varies across the resonance wavelengths of 650 nm, 770 nm, and 920 nm. The experimental SPR curves show good agreement with the results of a Lorentz absorbance model at the lower particle surface coverages investigated. The results demonstrate an almost twofold signal difference for a subset of the experimental conditions explored.
我们展示了在基于表面等离子体共振（SPR）的检测中，吸附在平面金表面的胶体金纳米颗粒的共振波长依赖性信号。针对三种不同的共振波长，给出了SPR信号随颗粒表面覆盖率变化的实验测量结果。由于胶体金纳米颗粒产生的SPR信号在650 nm、770 nm和920 nm的共振波长范围内有所不同。在所研究的较低颗粒表面覆盖率下，实验SPR曲线与洛伦兹吸光度模型的结果吻合良好。结果表明，在所探索的部分实验条件下，信号差异几乎达到两倍。
