Marsh Erica E, Lin Zhihong, Yin Ping, Milad Magdy, Chakravarti Debabrata, Bulun Serdar E
Division of Reproductive Biology Research, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
Fertil Steril. 2008 Jun;89(6):1771-6. doi: 10.1016/j.fertnstert.2007.05.074. Epub 2007 Sep 4.
To determine whether microRNAs (miRNAs) are differentially expressed in human leiomyoma versus matched myometrial tissue.
Microarray with real-time polymerase chain reaction (PCR) validation.
Academic medical center.
PATIENT(S): Premenopausal subjects (n = 15), who were undergoing hysterectomies for leiomyoma-related symptoms.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Statistically differential expression of miRNAs in leiomyoma versus myometrium.
RESULT(S): Forty-six miRNA species were differentially expressed in leiomyoma versus normal myometrium. Of these, 19 were overexpressed and 27 were underexpressed in leiomyomas. The changes ranged from 1.2-fold to 11.8-fold. These findings were confirmed using real-time reverse transcriptase PCR for selected miRNAs (miRNAs 21, 34a, 125b, 139, and 323).
CONCLUSION(S): Our findings indicate that miRNAs are differentially expressed between human leiomyoma and matched myometrium. Given this differential expression, miRNAs may play a role in the pathogenesis of uterine leiomyoma and may serve as future therapeutic targets for the treatment of these tumors.
确定微小RNA（miRNA）在人类平滑肌瘤与配对的子宫肌层组织中是否存在差异表达。
采用实时聚合酶链反应（PCR）验证的微阵列技术。
学术医学中心。
因平滑肌瘤相关症状接受子宫切除术的绝经前受试者（n = 15）。
无。
平滑肌瘤与子宫肌层中miRNA的统计学差异表达。
46种miRNA在平滑肌瘤与正常子宫肌层中存在差异表达。其中，19种在平滑肌瘤中过表达，27种在平滑肌瘤中低表达。变化范围为1.2倍至11.8倍。通过对选定的miRNA（miRNA 21、34a、125b、139和323）进行实时逆转录PCR，证实了这些发现。
我们的研究结果表明，miRNA在人类平滑肌瘤和配对的子宫肌层之间存在差异表达。鉴于这种差异表达，miRNA可能在子宫平滑肌瘤的发病机制中起作用，并可能成为未来治疗这些肿瘤的靶点。
