乳酸杆菌：在语音假体生物膜形成中起重要作用。

Lactobacilli: important in biofilm formation on voice prostheses.

作者信息

Buijssen Kevin J D A, Harmsen Hermie J M, van der Mei Henny C, Busscher Henk J, van der Laan Bernard F A M

机构信息

Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Groningen, the Netherlands.

出版信息

Otolaryngol Head Neck Surg. 2007 Sep;137(3):505-7. doi: 10.1016/j.otohns.2007.05.051.

DOI:10.1016/j.otohns.2007.05.051

PMID:17765785

Abstract

OBJECTIVE

We sought to identify bacterial strains responsible for biofilm formation on silicone rubber voice prostheses.

STUDY DESIGN

We conducted an analysis of the bacterial population in biofilms on used silicone rubber voice prostheses by using new microbiological methods.

METHODS

Two microbiological methods were used: polymerase chain reaction-denaturing gradient gel electrophoresis and fluorescence in situ hybridization. Twenty-six Provox2 and eight Groningen Ultra Low Resistance voice prostheses that were removed because of leakage through the prosthesis or because of increased airflow resistance were used in this study.

RESULTS

The results showed that 33 of the 34 explanted voice prosthetic biofilms contained lactobacilli in close association with the Candida sp. present.

CONCLUSION

Lactobacilli are general colonizers of tracheoesophageal voice prostheses in vivo, growing intertwined with Candida. This knowledge may be important in the development of new pathways directed to prevent or to influence biofilm formation on tracheoesophageal voice prostheses and elongate their lifespan.

摘要