Department of Otorhinolaryngology, Medical University of Vienna, Vienna, Austria.
Head Neck. 2010 Jul;32(7):886-95. doi: 10.1002/hed.21276.
In voice rehabilitation for laryngectomized patients, voice prosthetic biofilm formation is still an unsolved problem. Design and materials of voice prostheses have been altered by manufacturers to improve function and extend the lifetime of devices. The goal of the study was to investigate biofilm formation on Provox 2 and Phonax, recently introduced voice prostheses made of thermoplastic polyurethane.
Five laryngectomized patients were equipped with both Phonax and Provox 2 voice prostheses. Microbial colonization was analyzed using standard microbiological methods. Biofilm formation and material infiltration were illustrated using scanning electron microscopy, fluorescence microscopy, and thin-section light microscopy.
Although no differences in quality or quantity of microbial colonization were assessed, microscopic imaging revealed differences in material surfaces, biofilm composition, and infiltration morphologies; the polyurethane material seems to destabilize biofilm architecture by inhibition of hypheal Candida growth forms.
Polyurethane material for voice prostheses seems to reduce biofilm stability and infiltrative processes.
在喉切除患者的语音康复中，人工发声假体生物膜的形成仍然是一个未解决的问题。制造商已经改变了发声假体的设计和材料，以提高其功能并延长设备的使用寿命。本研究的目的是研究最近推出的两种热塑性聚氨酯发声假体——Provox 2 和 Phonax 上生物膜的形成。
为 5 名喉切除患者配备了 Phonax 和 Provox 2 两种发声假体。使用标准微生物学方法分析微生物定殖情况。使用扫描电子显微镜、荧光显微镜和薄切片光显微镜观察生物膜形成和材料渗透情况。
尽管评估的微生物定植质量和数量没有差异，但显微镜成像显示了材料表面、生物膜成分和渗透形态的差异；聚氨酯材料似乎通过抑制假丝酵母菌的菌丝生长形式来破坏生物膜结构的稳定性。
用于发声假体的聚氨酯材料似乎降低了生物膜的稳定性和渗透过程。
