Leunisse C, van Weissenbruch R, Busscher H J, van der Mei H C, Albers F W
Department of Otorhinolaryngology, University Hospital Groningen Hanzeplein 1, The Netherlands.
Acta Otolaryngol. 1999;119(5):604-8.
After total laryngectomy, the voice can be restored successfully with a silicone tracheo-oesophageal voice prosthesis. Biofilm formation and subsequent deterioration of the silicone material of the prosthesis often limit the mean life of the device to an average of 3-5 months. Although device replacement can be considered an easy outpatient procedure, frequent replacements are inconvenient for the patient and may lead to malfunction of the tracheo-oesophageal fistula. Further understanding of the process of development and inhibition of the colonization of these polymer surfaces requires several comprehensive clinical studies. However, in vivo research of the biomaterials of the voice prostheses is difficult and time consuming. In order to simulate the natural process of biofilm development under dynamic nutrient conditions, an artificial throat was developed. Biofilm developed on Groningen button voice prostheses in vitro could not be distinguished from that formed over several months in vivo. This method can be used as a standardized approach for studying functional and structural aspects of all commercially available indwelling and non-indwelling voice prostheses, including the Groningen button, Provox, Voice Master, Blom-Singer and others under various laboratory conditions.
全喉切除术后，使用硅胶气管食管发音假体可成功恢复嗓音。假体硅胶材料上生物膜的形成及随后的材料降解，常常将该装置的平均使用寿命限制在平均3至5个月。尽管装置更换可被视为一种简单的门诊手术，但频繁更换对患者来说不方便，且可能导致气管食管瘘出现故障。要进一步了解这些聚合物表面的定植发展及抑制过程，需要开展多项全面的临床研究。然而，对发音假体生物材料进行体内研究既困难又耗时。为了在动态营养条件下模拟生物膜形成的自然过程，研发了一种人工喉。在体外格罗宁根纽扣式发音假体上形成的生物膜与在体内数月内形成的生物膜并无差异。该方法可用作一种标准化手段，用于研究所有市售的植入式和非植入式发音假体的功能和结构方面，包括格罗宁根纽扣式、Provox、Voice Master、Blom - Singer等假体在各种实验室条件下的情况。
