Sheng-Hua Chu, Yan-Bin Ma, Zhi-An Zhu, Hong Zhang, Dong-Fu Feng, Zhi-Qiang Li, Xian-Hou Yuan
Department of Neurosurgery, No. 3 People's Hospital Affiliated to Shanghai Jiao Tong University College of Medicine, Shanghai 201900, ROC China.
Department of Neurosurgery, No. 3 People's Hospital Affiliated to Shanghai Jiao Tong University College of Medicine, Shanghai 201900, ROC China.
Surg Neurol. 2007 Dec;68(6):610-613. doi: 10.1016/j.surneu.2006.12.050. Epub 2007 Sep 4.
Postoperative radiotherapy is the standard treatment for patients with a malignant glioma. However, a malignant glioma is radioresistant and almost always recurs, even after a high dose of radiation. A malignant glioma is characterized by its proliferation, invasion and neoangiogenesis, which can be attributed to the high levels of HGF. The scope of this study is to investigate HGF secretion by malignant glioma cells with different radiosensitivity after irradiation.
Three human malignant glioma cell lines (U251, U251-NG2, and BT325) were irradiated with single doses of 0, 5, 10, and 20 grays of gamma-rays from a (137)Cs source. Hepatocyte growth factor levels in medium were measured by ELISA at 24, 48, and 72 hours after radiation. Cell survival was measured by the proliferation-based assay (XTT assay) 7 days after irradiation.
After a single dose radiation, the HGF levels showed a dose-dependent increase in U251, U251-NG2, and BT325 glioma cells. Both baseline and radiation-enhanced HGF levels were about 10-fold higher in BT325 compared to U251 and U251-NG2 cells. In addition, in the XTT assay, the BT325 was more radioresistant than both U251 and U251-NG2 cell lines (dose modifying factor = 1.5 and 1.6, respectively).
Irradiation-enhanced HGF secretion in all 3 tested glioma cell lines (up to 7 times basal levels). It is tempting to associate the radiation-enhanced HGF secretion with an increased angiogenic potential of the tumor, which may be a factor in radioresistance.
术后放疗是恶性胶质瘤患者的标准治疗方法。然而，恶性胶质瘤具有放射抗性，即使经过高剂量放疗，几乎总会复发。恶性胶质瘤的特征在于其增殖、侵袭和新生血管形成，这可归因于高水平的肝细胞生长因子（HGF）。本研究的范围是调查照射后具有不同放射敏感性的恶性胶质瘤细胞分泌HGF的情况。
三种人类恶性胶质瘤细胞系（U251、U251-NG2和BT325）用来自铯-137源的单剂量0、5、10和20格雷的γ射线进行照射。在放疗后24、48和72小时通过酶联免疫吸附测定（ELISA）测量培养基中的肝细胞生长因子水平。在照射7天后通过基于增殖的检测方法（XTT检测）测量细胞存活率。
单剂量放疗后，U251、U251-NG2和BT325胶质瘤细胞中的HGF水平呈剂量依赖性增加。与U251和U251-NG2细胞相比，BT325细胞的基线和放疗增强的HGF水平均高出约10倍。此外，在XTT检测中，BT325比U251和U251-NG2细胞系更具放射抗性（剂量修正因子分别为1.5和1.6）。
在所有3种测试的胶质瘤细胞系中，照射均增强了HGF分泌（高达基础水平的7倍）。很容易将照射增强的HGF分泌与肿瘤血管生成潜能增加联系起来，这可能是放射抗性的一个因素。
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